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Key=gene_full_name, value=Tyrosine-protein kinase SYK
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Key=description, value=The SYK (Phospho-Tyr348) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SYK (Phospho-Tyr348) protein expression profile in cells. The kit can be used for measuring the relative amounts of SYK (Phospho-Tyr348) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SYK (Phospho-Tyr348).
Key=short_description, value=The SYK (Phospho-Tyr348) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SYK (Phospho-Tyr348) protein expression profile in cells. The kit can be used for measuring the relative amounts of SYK (Phospho-Tyr348) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SYK (Phospho-Tyr348).
Key=gene_name, value=SYK
Key=synonyms, value=Spleen tyrosine kinase;PROTEIN-TYROSINE KINASE SYK
Key=sample_type, value=Cell lines
Key=kit_components, value=SYK (Phospho-Tyr348)
Key=subcellular_localization, value=Cell membrane. Cytoplasm, cytosol.
Key=tissue_specificity, value=Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.
Key=protein_function, value= Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine- phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T- cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. Together with CEACAM20, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response (By similarity).
Key=storage, value=Store at 4°C for up to 6 months.
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SYK (Phospho-Tyr348) Colorimetric Cell-Based ELISA Kit

SYK ELISA kit, phospho-specific, cell-based

The SYK (Phospho-Tyr348) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SYK (Phospho-Tyr348) protein expression profile in cells. The kit can be used for measuring the relative amounts of SYK (Phospho-Tyr348) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SYK (Phospho-Tyr348).

Product Info Summary

SKU: EKC2005
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

SYK (Phospho-Tyr348) Colorimetric Cell-Based ELISA Kit

See all SYK products

SKU/Catalog Number

EKC2005

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The SYK (Phospho-Tyr348) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SYK (Phospho-Tyr348) protein expression profile in cells. The kit can be used for measuring the relative amounts of SYK (Phospho-Tyr348) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SYK (Phospho-Tyr348).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

SYK (Phospho-Tyr348) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2005)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2005 is reactive to SYK in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2005 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-SYK Phospho-Tyr348 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-SYK Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For SYK (Source: Uniprot.Org, NCBI)

Gene Name

SYK

Full Name

Tyrosine-protein kinase SYK

Weight

72066 MW

Superfamily

protein kinase superfamily

Alternative Names

Spleen tyrosine kinase;PROTEIN-TYROSINE KINASE SYK SYK p72-Syk spleen associated tyrosine kinase tyrosine-protein kinase SYK|spleen tyrosine kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SYK, check out the SYK Infographic

SYK infographic

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In this infographic you will see the following information for SYK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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