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Product Info Summary
| SKU: | M00583 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-SUZ12 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00583
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SUZ12 Rabbit Monoclonal Antibody catalog # M00583. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SUZ12 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00583)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IEB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SUZ12
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00583 is reactive to SUZ12 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
83055 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00583 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: HeLa cell, SW480 cell
Validation Images & Assay Conditions
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Western blot analysis of SUZ12 expression in (1) HeLa cell lysate; (2) SW480 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For SUZ12 (Source: Uniprot.org, NCBI)
Gene Name
SUZ12
Full Name
Polycomb protein SUZ12
Weight
83055 MW
Superfamily
VEFS (VRN2-EMF2-FIS2-SU(Z)12) family
Alternative Names
Polycomb protein SUZ12;Chromatin precipitated E2F target 9 protein;ChET 9 protein;Joined to JAZF1 protein;Suppressor of zeste 12 protein homolog;SUZ12;CHET9, JJAZ1, KIAA0160; SUZ12 CHET9, IMMAS, JJAZ1 SUZ12 polycomb repressive complex 2 subunit polycomb protein SUZ12|chET 9 protein|chromatin precipitated E2F target 9 protein|joined to JAZF1 protein|suppressor of zeste 12 protein homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SUZ12, check out the SUZ12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SUZ12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SUZ12 Rabbit Monoclonal Antibody (M00583)
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No publications found for M00583
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Customer Q&As
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4 Customer Q&As for Anti-SUZ12 Rabbit Monoclonal Antibody
Question
Does anti-SUZ12 Rabbit Monoclonal antibody M00583 work on dog IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
Our lab technicians have not tested anti-SUZ12 Rabbit Monoclonal antibody M00583 on dog. You can run a BLAST between dog and the immunogen sequence of anti-SUZ12 Rabbit Monoclonal antibody M00583 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog leukemic t-cell in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-09
Question
Do you have a BSA free version of anti-SUZ12 Rabbit Monoclonal antibody M00583 available?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SUZ12 Rabbit Monoclonal antibody M00583 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-02
Question
Would M00583 anti-SUZ12 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
It shows on the product datasheet, M00583 anti-SUZ12 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-01
Question
My question regarding product M00583, anti-SUZ12 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00583 anti-SUZ12 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-18
