Anti-FBXL11/KDM2A Antibody Picoband®

Product Name

Anti-FBXL11/KDM2A Antibody Picoband®

View all KDM2A/FBXL11 Antibodies

SKU/Catalog Number

A03027-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FBXL11/KDM2A Antibody Picoband® catalog # A03027-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FBXL11/KDM2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03027-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human FBXL11, which shares 97.1% and 100% amino acid (aa) sequence identity with mouse and rat FBXL11, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03027-1 is reactive to KDM2A in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

150 kDa

Calculated molecular weight

132.793kDa

Background of KDM2A/FBXL11

Lysine-specific demethylase 2A (KDM2A) also known as F-box and leucine-rich repeat protein 11 (FBXL11) is an enzyme that in humans is encoded by the KDM2A gene. This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03027-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Positive Control

WB: mouse liver tissue, human A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For KDM2A (Source: Uniprot.org, NCBI)

Alternative Names

Lysine-specific demethylase 2A; CXXC-type zinc finger protein 8; F-box and leucine-rich repeat protein 11; F-box protein FBL7; F-box protein Lilina; F-box/LRR-repeat protein 11; JmjC domain-containing histone demethylation protein 1A; [Histone-H3]-lysine-36 demethylase 1A; KDM2A; CXXC8; FBL7; FBXL11; JHDM1A; KIAA1004 KDM2A CXXC8, FBL11, FBL7, FBXL11, JHDM1A, LILINA lysine demethylase 2A lysine-specific demethylase 2A|CXXC-type zinc finger protein 8|F-box and leucine-rich repeat protein 11|F-box/LRR-repeat protein 11|[Histone-H3]-lysine-36 demethylase 1A|jmjC domain-containing histone demethylation protein 1A|jumonji C domain-containing histone demethylase 1A|lysine (K)-specific demethylase 2A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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