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Product Info Summary
| SKU: | PB9822 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SULT2B1 Antibody Picoband®
View all Cytosolic Sulfotransferase 2B1/SULT2B1 Antibodies
SKU/Catalog Number
PB9822
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SULT2B1 Antibody Picoband® catalog # PB9822. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SULT2B1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9822)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SULT2B1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9822 is reactive to SULT2B1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
41308 MW
Background of Cytosolic Sulfotransferase 2B1/SULT2B1
Sulfotransferase family cytosolic 2B member 1 is an enzyme that in humans is encoded by the SULT2B1 gene. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. And this gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9822 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: Rat Testis Tissue, A431 Whole Cell, HELA Whole Cell, MCF-7 Whole Cell
IHC: rat intestine tissue, human intestinal cancer tissue
ICC/IF: A431 cell
FCM: A549 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Testis Tissue Lysate at 50ug,
Lane 2: A431 Whole Cell Lysate at 40ug,
Lane 3: HELA Whole Cell Lysate at 40ug,
Lane 4: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2B1 antigen affinity purified polyclonal antibody (Catalog # PB9822) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2B1 at approximately 48 kDa. The expected band size for SULT2B1 is at 48 kDa.
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Figure 2. IHC analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822).
SULT2B1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2B1 Antibody (PB9822) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822).
SULT2B1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2B1 Antibody (PB9822) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822).
SULT2B1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SULT2B1 Antibody (PB9822) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 5. Flow Cytometry analysis of A549 cells using anti-SULT2B1 antibody (PB9822).
Overlay histogram showing A549 cells stained with PB9822 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SULT2B1 Antibody (PB9822,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SULT2B1 (Source: Uniprot.org, NCBI)
Gene Name
SULT2B1
Full Name
Sulfotransferase 2B1
Weight
41308 MW
Superfamily
sulfotransferase 1 family
Alternative Names
Sulfotransferase family cytosolic 2B member 1;ST2B1;Sulfotransferase 2B1;2.8.2.2;Alcohol sulfotransferase;Hydroxysteroid sulfotransferase 2;SULT2B1;HSST2; SULT2B1 ARCI14, HSST2 sulfotransferase family 2B member 1 sulfotransferase 2B1|ST2B1|alcohol sulfotransferase|hydroxysteroid sulfotransferase 2|hydroxysteroid sulfotransferase SULT2B1a|sulfotransferase family, cytosolic, 2B, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SULT2B1, check out the SULT2B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SULT2B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SULT2B1 Antibody Picoband® (PB9822)
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Customer Q&As
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6 Customer Q&As for Anti-SULT2B1 Antibody Picoband®
Question
We want to test anti-SULT2B1 antibody PB9822 on rat placenta for research purposes, then I may be interested in using anti-SULT2B1 antibody PB9822 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The products we sell, including anti-SULT2B1 antibody PB9822, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-21
Question
Is a blocking peptide available for product anti-SULT2B1 antibody (PB9822)?
O. Wu
Verified customer
Asked: 2019-08-23
Answer
We do provide the blocking peptide for product anti-SULT2B1 antibody (PB9822). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-23
Question
I was wanting to use your anti-SULT2B1 antibody for WB for rat placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat placenta identification?
K. Taylor
Verified customer
Asked: 2017-11-10
Answer
It shows on the product datasheet, PB9822 anti-SULT2B1 antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-10
Question
Will PB9822 anti-SULT2B1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
As indicated on the product datasheet, PB9822 anti-SULT2B1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-SULT2B1 antibody PB9822. Let me know if you need anything else.
W. Singh
Verified customer
Asked: 2017-02-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-03
Question
We are currently using anti-SULT2B1 antibody PB9822 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on feline tissues as well?
R. Wu
Verified customer
Asked: 2015-12-17
Answer
The anti-SULT2B1 antibody (PB9822) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-17
