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Product Info Summary
| SKU: | PB9821 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SULT2A1 Antibody Picoband®
View all Cytosolic Sulfotransferase 2A1/SULT2A1 Antibodies
SKU/Catalog Number
PB9821
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SULT2A1 Antibody Picoband® catalog # PB9821. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SULT2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9821)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SULT2A1, different from the related mouse sequence by seven amino acids, and from the related rat sequence by eight amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9821 is reactive to SULT2A1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30-35 kDa
Calculated molecular weight
33780 MW
Background of Cytosolic Sulfotransferase 2A1/SULT2A1
Bile salt sulfotransferase, also known as hydroxysteroid sulfotransferase (HST) or sulfotransferase 2A1 (ST2A1), is an enzyme that in humans is encoded by the SULT2A1 gene. It is mapped to 19q13.3. This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9821 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
WB: human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP), human HepG2 whole cell, rat liver tissue
IHC: mouse liver tissue, rat liver tissue, human renal cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2A1 antigen affinity purified polyclonal antibody (Catalog # PB9821) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2A1 at approximately 30-35 kDa. The expected band size for SULT2A1 is at 34 kDa.
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Figure 2. IHC analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821).
SULT2A1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2A1 Antibody (PB9821) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821).
SULT2A1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2A1 Antibody (PB9821) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821).
SULT2A1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2A1 Antibody (PB9821) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SULT2A1 (Source: Uniprot.org, NCBI)
Gene Name
SULT2A1
Full Name
Bile salt sulfotransferase
Weight
33780 MW
Superfamily
sulfotransferase 1 family
Alternative Names
Bile salt sulfotransferase;2.8.2.14;Dehydroepiandrosterone sulfotransferase;DHEA-ST;Hydroxysteroid Sulfotransferase;HST;ST2;ST2A3;Sulfotransferase 2A1;ST2A1;SULT2A1;HST, STD; SULT2A1 DHEA-ST, DHEA-ST8, DHEAS, HST, ST2, ST2A1, ST2A3, STD, SULT2A3, hSTa sulfotransferase family 2A member 1 sulfotransferase 2A1|alcohol/hydroxysteroid sulfotransferase|bile-salt sulfotranasferase 2A1|sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SULT2A1, check out the SULT2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SULT2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SULT2A1 Antibody Picoband® (PB9821)
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No publications found for PB9821
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Customer Q&As
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4 Customer Q&As for Anti-SULT2A1 Antibody Picoband®
Question
I have attached the WB image, lot number and protocol we used for adrenal gland using anti-SULT2A1 antibody PB9821. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-27
Question
Is there a BSA free version of anti-SULT2A1 antibody PB9821 available?
Verified Customer
Verified customer
Asked: 2018-09-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SULT2A1 antibody PB9821 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-05
Question
Would anti-SULT2A1 antibody PB9821 work for IHC with adrenal gland?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
According to the expression profile of adrenal gland, SULT2A1 is highly expressed in adrenal gland. So, it is likely that anti-SULT2A1 antibody PB9821 will work for IHC with adrenal gland.
Boster Scientific Support
Answered: 2017-06-27
Question
We are currently using anti-SULT2A1 antibody PB9821 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
C. Lewis
Verified customer
Asked: 2016-09-27
Answer
The anti-SULT2A1 antibody (PB9821) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-27
