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Product Info Summary
| SKU: | M00312 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Stromal interaction molecule 1 STIM1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00312
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Stromal interaction molecule 1 STIM1 Rabbit Monoclonal Antibody catalog # M00312. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Stromal interaction molecule 1 STIM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00312)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HEC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Stromal interaction molecule 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00312 is reactive to STIM1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
77423 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00312 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: human Jurkat whole cell, human K562 whole cell, human HepG2 whole cell, human HUH-7 whole cell, rat RH35 whole cell, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STIM1 using anti-STIM1 antibody (M00312).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HUH-7 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM1 antigen affinity purified monoclonal antibody (Catalog # M00312) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIM1 at approximately 85 kDa. The expected band size for STIM1 is at 77 kDa.
Protein Target Info & Infographic
Gene/Protein Information For STIM1 (Source: Uniprot.org, NCBI)
Gene Name
STIM1
Full Name
Stromal interaction molecule 1
Weight
77423 MW
Alternative Names
Stromal interaction molecule 1;STIM1;GOK; STIM1 D11S4896E, GOK, IMD10, STRMK, TAM, TAM1 stromal interaction molecule 1 stromal interaction molecule 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STIM1, check out the STIM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STIM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Stromal interaction molecule 1 STIM1 Rabbit Monoclonal Antibody (M00312)
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No publications found for M00312
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Customer Q&As
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16 Customer Q&As for Anti-Stromal interaction molecule 1 STIM1 Rabbit Monoclonal Antibody
Question
Is this M00312 anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody reactive to the isotypes of STIM1?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The immunogen of M00312 anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Stromal interaction molecule 1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-30
Question
We have tried in the past anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody for IP on liver in a previous project. I am using human, and We are going to use the antibody for WB next. I am interested in examining liver as well as fetal liver placenta in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
I took a look at the website and datasheets of our anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody and it seems that M00312 has been tested on human in both IP and WB. Thus M00312 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-27
Question
My question regarding product M00312, anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00312 anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-13
Question
Does M00312 anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
You can see on the product datasheet, M00312 anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-19
Question
I was wanting to use your anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody for IP for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
You can see on the product datasheet, M00312 anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-26
Question
Our lab were well pleased with the WB result of your anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody. However we have seen positive staining in gastrocnemius cell membrane using this antibody. Is that expected? Could you tell me where is STIM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
According to literature, gastrocnemius does express STIM1. Generally STIM1 expresses in cell membrane. Regarding which tissues have STIM1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal liver, and Placenta, Pubmed ID: 8921403
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Pancreas, Pubmed ID: 15489334
Platelet, Pubmed ID: 16263699, 18088087
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-08-21
Question
My question regards to test anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312 on human t-cell for research purposes, then I may be interested in using anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
The products we sell, including anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-22
Question
Is there a BSA free version of anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312 available?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-17
Question
See below the WB image, lot number and protocol we used for t-cell using anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-12
Question
We have been able to see staining in human gastrocnemius. What should we do? Is anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody supposed to stain gastrocnemius positively?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
According to literature gastrocnemius does express STIM1. According to Uniprot.org, STIM1 is expressed in gastrocnemius, fetal liver placenta, pancreas, cervix carcinoma, t-cell, platelet, leukemic t-cell, liver, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have STIM1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal liver, and Placenta, Pubmed ID: 8921403
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Pancreas, Pubmed ID: 15489334
Platelet, Pubmed ID: 16263699, 18088087
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2018-09-19
Question
Will anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312 work for IP with t-cell?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
According to the expression profile of t-cell, STIM1 is highly expressed in t-cell. So, it is likely that anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312 will work for IP with t-cell.
Boster Scientific Support
Answered: 2018-08-08
Question
Is a blocking peptide available for product anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody (M00312)?
J. Jackson
Verified customer
Asked: 2017-11-03
Answer
We do provide the blocking peptide for product anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody (M00312). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-03
Question
I am interested in using your anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody for regulation of cardiac conduction studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
J. Parker
Verified customer
Asked: 2017-09-29
Answer
I appreciate your inquiry. This M00312 anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-29
Question
We are currently using anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
L. Jones
Verified customer
Asked: 2017-03-28
Answer
The anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody (M00312) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-28
Question
I see that the anti-Stromal interaction molecule 1 Rabbit Monoclonal antibody M00312 works with IP, what is the protocol used to produce the result images on the product page?
L. Jones
Verified customer
Asked: 2016-04-15
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-04-15
