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Product Info Summary
| SKU: | PA1857 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Orai1 Antibody Picoband®
SKU/Catalog Number
PA1857
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Orai1 Antibody catalog # PA1857. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Orai1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1857)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Orai1, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1857 is reactive to ORAI1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
32668 MW
Background of ORAI1
ORAI1 (ORAI calcium release-activated calcium modulator 1), also known as CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selective ion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency (SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al. (2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion. Prakriya et al. (2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1857 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: SKOV Whole Cell
Validation Images & Assay Conditions
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Anti-Orai1 antibody, PA1857, Western blotting
All lanes: Anti Orai1 (PA1857) at 0.5ug/ml
WB: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 50KD
Protein Target Info & Infographic
Gene/Protein Information For ORAI1 (Source: Uniprot.org, NCBI)
Gene Name
ORAI1
Full Name
Calcium release-activated calcium channel protein 1
Weight
32668 MW
Superfamily
Orai family
Alternative Names
Calcium release-activated calcium channel protein 1;Protein orai-1;Transmembrane protein 142A;ORAI1;CRACM1, TMEM142A; ORAI1 CRACM1, IMD9, ORAT1, TAM2, TMEM142A ORAI calcium release-activated calcium modulator 1 calcium release-activated calcium channel protein 1|calcium release-activated calcium modulator 1|protein orai-1|transmembrane protein 142A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ORAI1, check out the ORAI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ORAI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Orai1 Antibody Picoband® (PA1857)
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No publications found for PA1857
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Customer Q&As
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5 Customer Q&As for Anti-Orai1 Antibody Picoband®
Question
My boss were well pleased with the WB result of your anti-Orai1 antibody. However we have been able to see positive staining in muscle of leg cell membrane using this antibody. Is that expected? Could you tell me where is ORAI1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
According to literature, muscle of leg does express ORAI1. Generally ORAI1 expresses in cell membrane. Regarding which tissues have ORAI1 expression, here are a few articles citing expression in various tissues:
Bone marrow, Ovary, Prostate, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648
Liver, Pubmed ID: 24275569
Mammary gland, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-27
Question
Would PA1857 work on mouse and rat samples?
Verified customer
Asked: 2019-09-12
Answer
We tested the Anti-Orai1 Antibody (PA1857) on mouse and rat samples and it didn't work well.
Boster Scientific Support
Answered: 2019-09-12
Question
I would like using your anti-Orai1 antibody for mammary gland epithelium development studies. Has this antibody been tested with western blotting on skov whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
We appreciate your inquiry. This PA1857 anti-Orai1 antibody is validated on skov whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-20
Question
We have been able to see staining in human ovary. What should we do? Is anti-Orai1 antibody supposed to stain ovary positively?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
From what I have seen in literature ovary does express ORAI1. From what I have seen in Uniprot.org, ORAI1 is expressed in muscle of leg, bone marrow, ovary, prostate uterus, mammary gland, cervix carcinoma, liver, among other tissues. Regarding which tissues have ORAI1 expression, here are a few articles citing expression in various tissues:
Bone marrow, Ovary, Prostate, and Uterus, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648
Liver, Pubmed ID: 24275569
Mammary gland, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-10-22
Question
We are currently using anti-Orai1 antibody PA1857 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
H. Krishna
Verified customer
Asked: 2013-01-09
Answer
The anti-Orai1 antibody (PA1857) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-09
