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Product Info Summary
| SKU: | PB9406 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-STIM1 Antibody Picoband®
SKU/Catalog Number
PB9406
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STIM1 Antibody Picoband® catalog # PB9406. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STIM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9406)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human STIM1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9406 is reactive to STIM1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
97 kDa
Calculated molecular weight
77423 MW
Background of STIM1
Stromal interaction molecule 1 is a protein that in humans is encoded by the STIM1 gene. STIM1 has a single transmembranedomain, and is localized to the endoplasmic reticulum, and to a lesser extent to the plasma membrane. This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9406 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Jurakt whole cell, human K562 whole cell, human HepG2 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue
IHC: mouse intestine tissue, rat intestine tissue, human mammary cancer tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STIM1 using anti-STIM1 antibody (PB9406, Left) and anti-STIM1 antibody (M00312, Right).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.
Lane 1: human Jurakt whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody (Catalog # PB9406) and rabbit anti-STIM1 antigen affinity purified monoclonal antibody (Catalog # (M00312) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIM1 at approximately 97KD. The expected band size for STIM1 is at 77KD.
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Figure 2. IHC analysis of STIM1 using anti-STIM1 antibody (PB9406).
STIM1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-STIM1 Antibody (PB9406) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of STIM1 using anti-STIM1 antibody (PB9406).
STIM1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-STIM1 Antibody (PB9406) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of STIM1 using anti-STIM1 antibody (PB9406).
STIM1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-STIM1 Antibody (PB9406) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 5. IF analysis of STIM1 using anti-STIM1 antibody (PB9406).
STIM1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-STIM1 Antibody (PB9406) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For STIM1 (Source: Uniprot.org, NCBI)
Gene Name
STIM1
Full Name
Stromal interaction molecule 1
Weight
77423 MW
Alternative Names
Stromal interaction molecule 1;STIM1;GOK; STIM1 D11S4896E, GOK, IMD10, STRMK, TAM, TAM1 stromal interaction molecule 1 stromal interaction molecule 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STIM1, check out the STIM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STIM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STIM1 Antibody Picoband® (PB9406)
Hello CJ!
PB9406 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Calcium dysregulation, functional calpainopathy, and endoplasmic reticulum stress in sporadic inclusion body myositis
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Customer Q&As
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5 Customer Q&As for Anti-STIM1 Antibody Picoband®
Question
We ordered your anti-STIM1 antibody for WB on t-cell a few years ago. I am using mouse, and We intend to use the antibody for IHC next. We want examining t-cell as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
I looked at the website and datasheets of our anti-STIM1 antibody and it seems that PB9406 has been tested on mouse in both WB and IHC. Thus PB9406 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-03
Question
We have been able to see staining in rat fetal liver placenta. Any tips? Is anti-STIM1 antibody supposed to stain fetal liver placenta positively?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Based on literature fetal liver placenta does express STIM1. Based on Uniprot.org, STIM1 is expressed in gastrocnemius, fetal liver placenta, pancreas, cervix carcinoma, t-cell, platelet, leukemic t-cell, liver, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have STIM1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal liver, and Placenta, Pubmed ID: 8921403
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Pancreas, Pubmed ID: 15489334
Platelet, Pubmed ID: 16263699, 18088087
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-11-19
Question
We are currently using anti-STIM1 antibody PB9406 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The anti-STIM1 antibody (PB9406) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-13
Question
I am interested in using your anti-STIM1 antibody for antigen activates b cell receptor (bcr) leading to generation of second messengers studies. Has this antibody been tested with western blotting on intestine tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
We appreciate your inquiry. This PB9406 anti-STIM1 antibody is tested on rat liver tissue, tissue lysate, mouse liver tissue, human placenta tissue, hela whole cell lysate, smmc whole cell lysate, hepg2 whole cell lysate, intestine tissue, mammary cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-19
Question
My boss were satisfied with the WB result of your anti-STIM1 antibody. However we have observed positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is STIM1 supposed to be expressed?
S. Li
Verified customer
Asked: 2016-06-06
Answer
According to literature, leukemic t-cell does express STIM1. Generally STIM1 expresses in cell membrane. Regarding which tissues have STIM1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal liver, and Placenta, Pubmed ID: 8921403
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Pancreas, Pubmed ID: 15489334
Platelet, Pubmed ID: 16263699, 18088087
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2016-06-06
