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Product Info Summary
| SKU: | MP01087 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-STAT5 phospho Y694 STAT5A Monoclonal Antibody
SKU/Catalog Number
MP01087
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-STAT5 phospho Y694 STAT5A Monoclonal Antibody (Catalog # MP01087). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store STAT5 phospho Y694 Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 antibody is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-STAT5 phospho Y694 STAT5A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP01087)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 5F6.F1
Isotype
IgG1 kappa
Immunogen
Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
MP01087 is reactive to STAT5A in Human, Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
496341 MW
Background of Stat5a
Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5α and Stat5ß. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MP01087 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000
IHC: 20-40 µg/ml
IF Microscopy: 1:50-1:1000
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody(1:500) detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy).Red represents Stat5 pY694 protein.Green represents tubulin.
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Figure 1. IHC analysis of STAT5A using anti-STAT5A antibody (MP01087).
STAT5A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-STAT5A Antibody (MP01087) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 2. Western blot analysis of STAT5A using anti-STAT5A antibody (MP01087).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A antigen affinity purified polyclonal antibody (Catalog # MP01087) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for STAT5A.
Protein Target Info & Infographic
Gene/Protein Information For STAT5A (Source: Uniprot.org, NCBI)
Gene Name
STAT5A
Full Name
Signal transducer and activator of transcription 5A
Weight
496341 MW
Superfamily
transcription factor STAT family
Alternative Names
STAT5 pY694, MGF, Signal Transducer and Activator of Transcription 5A, STAT 5 STAT5A MGF, STAT5 signal transducer and activator of transcription 5A signal transducer and activator of transcription 5A|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT5A, check out the STAT5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT5 phospho Y694 STAT5A Monoclonal Antibody (MP01087)
Hello CJ!
MP01087 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hu T, Zhang C, Tang Q, Su Y, Li B, Chen L, Zhang Z, Cai T, Zhu Y. Bmc Cancer. 2013 May 22;13:251. Doi: 10.1186/1471-2407-13-251. Variant G6Pd Levels Promote Tumor Cell Proliferation Or Apoptosis Via The Stat3/5 Pathway In The Human Melanoma Xenogr...
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