Click image to see more details
Product Info Summary
| SKU: | P01087-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody
SKU/Catalog Number
P01087-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody catalog # P01087-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01087-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EDD-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Stat5 (Y694)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01087-1 is reactive to STAT5A in Human, Mouse
Reconstitution
Observed Molecular Weight
95 kDa
Calculated molecular weight
90647 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01087-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human thyroid tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of STAT5A using anti-STAT5A antibody (P01087-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A antigen affinity purified monoclonal antibody (Catalog # P01087-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A at approximately 95 kDa. The expected band size for STAT5A is at 91 kDa.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human thyroid, using Phospho-Stat5 (Y694) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For STAT5A (Source: Uniprot.org, NCBI)
Gene Name
STAT5A
Full Name
Signal transducer and activator of transcription 5A
Weight
90647 MW
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 5A;STAT5A;STAT5; STAT5A MGF, STAT5 signal transducer and activator of transcription 5A signal transducer and activator of transcription 5A|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT5A, check out the STAT5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody (P01087-1)
Hello CJ!
P01087-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hu T, Zhang C, Tang Q, Su Y, Li B, Chen L, Zhang Z, Cai T, Zhu Y. Bmc Cancer. 2013 May 22;13:251. Doi: 10.1186/1471-2407-13-251. Variant G6Pd Levels Promote Tumor Cell Proliferation Or Apoptosis Via The Stat3/5 Pathway In The Human Melanoma Xenogr...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
