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Product Info Summary
| SKU: | PA1692 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-STAT4 Antibody Picoband®
SKU/Catalog Number
PA1692
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STAT4 Antibody catalog # PA1692. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1692)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human STAT4, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1692 is reactive to STAT4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
12 kDa
Calculated molecular weight
85941 MW
Background of STAT4
STAT4 is a human gene. The protein encoded by this gene is a member of the STAT protein family of transcription factors. STAT4 is mapped to chromosome 2q32.2-q32.3 by fluorescence in situ hybridization. STAT4 is phosphorylated in response to interleukin-12 and is essential for IL12 signal transduction. Transactivation, electrophoretic mobility shift, and RNA interference analyses showed that Ikaros bound to the STAT4 promoter and was involved in regulation of STAT4 in human T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1692 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis Tissue, U87 Cell, HELA Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-STAT4 antibody, PA1692, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: U87 Cell Lysate
Lane 3: HELA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For STAT4 (Source: Uniprot.org, NCBI)
Gene Name
STAT4
Full Name
Signal transducer and activator of transcription 4
Weight
85941 MW
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 4;STAT4; STAT4 SLEB11 signal transducer and activator of transcription 4 signal transducer and activator of transcription 4|signal transducer and activator of transcription 4 variant 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT4, check out the STAT4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT4 Antibody Picoband® (PA1692)
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No publications found for PA1692
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-STAT4 Antibody Picoband®
Question
Can you help my question with product PA1692, anti-STAT4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1692 anti-STAT4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-17
Question
Here is the WB image, lot number and protocol we used for testis using anti-STAT4 antibody PA1692. Please let me know if you require anything else.
B. Jones
Verified customer
Asked: 2019-11-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-01
Question
Is this PA1692 anti-STAT4 antibody reactive to the isotypes of STAT4?
D. Edwards
Verified customer
Asked: 2018-08-28
Answer
The immunogen of PA1692 anti-STAT4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human STAT4(1-17aa MSQWNQVQQLEIKFLEQ), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-28
Question
We are currently using anti-STAT4 antibody PA1692 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
D. Carter
Verified customer
Asked: 2013-09-20
Answer
The anti-STAT4 antibody (PA1692) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-20
