Product Info Summary
SKU: | RC1040 |
---|---|
Size: | 1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO. |
Application: | Functional Assay |
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Product info
Product Name
STAT4 Luciferase Reporter-HEK293 Cell Line
SKU/Catalog Number
RC1040
Size
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Description
The STAT4 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a member of the STAT transcription factor family and plays a central role in generating inflammation during protective immune responses and immune-mediated diseases. The STAT4 induction by interferon gamma is shown in Figure 1.
Contents
Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Gene Name
STAT4
Storage & Handling
Immediately upon receipt, store in liquid nitrogen. (Ship on dry ice.)
Cite This Product
STAT4 Luciferase Reporter-HEK293 Cell Line (Boster Biological Technology, Pleasanton CA, USA, Catalog # RC1040)
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Application:
Monitor the STAT4 signaling pathway activity.Screen for activators or inhibitors of the STAT4 signaling pathway.Culture conditions:
Cells should be grown at 37°C with 5% CO2 using DMEM medium supplemented with 10% FBS and 1% Pen/Strep, plus 3 µg/ml of Puromycin. It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37°C water-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in 37°C-CO2 incubator. Leave the T25 flask in the incubator for 2~4 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence. To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.Functional validation:
A. Response of STAT4 HEK293 cells to interferon gamma (Figure 1).1. Harvest STAT4 HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 µl of growth medium at 5 x 104 cells/well. 2. Incubate cells at 37°C in a CO2 incubator for overnight. 3. The next day, stimulate cells with different concentrations of IFN-gamma. 4. Incubate at 37°C in a CO2 incubator for 6-16 hours. 5. Add 50 µl of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.Validation Images & Assay Conditions
![rc1040 stat4_hek293 rc1040 stat4_hek293](https://www.bosterbio.com/media/catalog/product/r/c/rc1040-stat4_hek293.jpg)
Click image to see more details
Fig-1: Induction of STAT4 activity by IFN-gamma in STAT4 HEK293 cells.
Protein Target Info & Infographic
Gene/Protein Information For STAT4 (Source: Uniprot.Org, NCBI)
Gene Name
STAT4
Full Name
Signal transducer and activator of transcription 4
Weight
Superfamily
transcription factor STAT family
Alternative Names
signal transducer and activator of transcription 4; SLEB11; STAT4 STAT4 SLEB11 signal transducer and activator of transcription 4 signal transducer and activator of transcription 4|signal transducer and activator of transcription 4 variant 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on STAT4, check out the STAT4 Infographic
![STAT4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for STAT4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For STAT4 Luciferase Reporter-HEK293 Cell Line (RC1040)
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