Anti-FOXP3 (Forkhead Box Protein P3) / Scurfin Monoclonal Antibody

Product Name

Anti-FOXP3 (Forkhead Box Protein P3) / Scurfin Monoclonal Antibody

View all Foxp3 Antibodies

SKU/Catalog Number

M00011

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-FOXP3 (Forkhead Box Protein P3) / Scurfin Monoclonal Antibody (Catalog # M00011). Tested in Flow Cytometry, WB, IHC applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-FOXP3 (Forkhead Box Protein P3) / Scurfin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00011)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM579

Isotype

IgG1, kappa

Immunogen

Full-length human FOXP3 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00011 is reactive to FOXP3 in Human, Monkey, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

47244 MW

Background of Foxp3

Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00011 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For FOXP3 (Source: Uniprot.org, NCBI)

Alternative Names

Forkhead box protein P3;Scurfin;Forkhead box protein P3, C-terminally processed;Forkhead box protein P3 41 kDa form;FOXP3;IPEX;JM2; Foxp3|JM2, scu, scurfin, sf|forkhead box P3|forkhead box protein P3|scurfy

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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