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Product Info Summary
| SKU: | RP1032 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Forkhead box protein P3 FOXP3 Antibody
SKU/Catalog Number
RP1032
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Forkhead box protein P3 FOXP3 Antibody catalog # RP1032. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Forkhead box protein P3 FOXP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1032)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOXP3 recombinant protein (Position: H101-P431). Human FOXP3 shares 88% amino acid (aa) sequence identity with mouse FOXP3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1032 is reactive to FOXP3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
47244 MW
Background of Foxp3
FOXP3 (forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome (specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1032 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
IHC: Human Tonsil Tissue, Mouse Spleen Tissue, Rat Spleen Tissue
Validation Images & Assay Conditions
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Anti-FOXP3 Picoband antibody, RP1032-1.JPG
IHC(P): Human Tonsil Tissue
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Anti-FOXP3 Picoband antibody, RP1032-2.JPG
IHC(P): Mouse Spleen Tissue
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Anti-FOXP3 Picoband antibody, RP1032-3.JPG
IHC(P):Rat Spleen Tissue
Protein Target Info & Infographic
Gene/Protein Information For FOXP3 (Source: Uniprot.org, NCBI)
Gene Name
FOXP3
Full Name
Forkhead box protein P3
Weight
47244 MW
Alternative Names
Forkhead box protein P3;Scurfin;Forkhead box protein P3, C-terminally processed;Forkhead box protein P3 41 kDa form;FOXP3;IPEX;JM2; Foxp3|JM2, scu, scurfin, sf|forkhead box P3|forkhead box protein P3|scurfy
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXP3, check out the FOXP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Forkhead box protein P3 FOXP3 Antibody (RP1032)
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Customer Q&As
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14 Customer Q&As for Anti-Forkhead box protein P3 FOXP3 Antibody
Question
Is a blocking peptide available for product anti-FOXP3 antibody (RP1032)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We do provide the blocking peptide for product anti-FOXP3 antibody (RP1032). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-06
Question
Would RP1032 anti-FOXP3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
It shows on the product datasheet, RP1032 anti-FOXP3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-FOXP3 antibody RP1032 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The anti-FOXP3 antibody (RP1032) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-25
Question
I was wanting to use your anti-FOXP3 antibody for IHC for mouse amniotic fluid on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse amniotic fluid identification?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
As indicated on the product datasheet, RP1032 anti-FOXP3 antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse amniotic fluid in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-10
Question
I have a question about product RP1032, anti-FOXP3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1032 anti-FOXP3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-FOXP3 antibody RP1032. Let me know if you need anything else.
J. Li
Verified customer
Asked: 2019-04-17
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-17
Question
you antibody using your anti-FOXP3 antibody for negative regulation of interleukin-2 production studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
A. Kulkarni
Verified customer
Asked: 2018-11-20
Answer
Thank you for your inquiry. This RP1032 anti-FOXP3 antibody is validated on human tonsil tissue, mouse spleen tissue. It is guaranteed to work for IHC in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-20
Question
Is there a BSA free version of anti-FOXP3 antibody RP1032 available?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-FOXP3 antibody RP1032 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-30
Question
I see that the anti-FOXP3 antibody RP1032 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-28
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-28
Question
I would like to test anti-FOXP3 antibody RP1032 on mouse amniotic fluid for research purposes, then I may be interested in using anti-FOXP3 antibody RP1032 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The products we sell, including anti-FOXP3 antibody RP1032, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-19
Question
Does anti-FOXP3 antibody RP1032 work on pig IHC with amniotic fluid?
A. Jackson
Verified customer
Asked: 2014-12-31
Answer
Our lab technicians have not validated anti-FOXP3 antibody RP1032 on pig. You can run a BLAST between pig and the immunogen sequence of anti-FOXP3 antibody RP1032 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig amniotic fluid in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-31
Question
Would anti-FOXP3 antibody RP1032 work for IHC with amniotic fluid?
A. Carter
Verified customer
Asked: 2014-12-17
Answer
According to the expression profile of amniotic fluid, FOXP3 is highly expressed in amniotic fluid. So, it is likely that anti-FOXP3 antibody RP1032 will work for IHC with amniotic fluid.
Boster Scientific Support
Answered: 2014-12-17
Question
Is this RP1032 anti-FOXP3 antibody reactive to the isotypes of FOXP3?
D. Jones
Verified customer
Asked: 2014-03-21
Answer
The immunogen of RP1032 anti-FOXP3 antibody is E.coli-derived human FOXP3 recombinant protein (Position: H101-P431). Human FOXP3 shares 88% amino acid (aa) sequence identity with mouse FOXP3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-03-21
Question
I have attached the WB image, lot number and protocol we used for amniotic fluid using anti-FOXP3 antibody RP1032. Please let me know if you require anything else.
L. Rodriguez
Verified customer
Asked: 2013-01-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-25
