Click image to see more details
Product Info Summary
| SKU: | M00036 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-STAT1 alpha Rabbit Monoclonal Antibody
SKU/Catalog Number
M00036
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-STAT1 alpha Rabbit Monoclonal Antibody catalog # M00036. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT1 alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00036)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HBG-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human STAT1 alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00036 is reactive to STAT1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
91 kDa
Calculated molecular weight
91 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00036 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:500
Positive Control
WB: human SiHa whole cell, human A549 whole cell, human Caco-2 whole cell, human PC-3 whole cell, human A431 whole cell, human MCF-7 whole cell, human RT4 whole cell, human HEL whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of STAT1 using anti-STAT1 antibody (M00036).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human MCF-7 whole cell lysates,
Lane 7: human RT4 whole cell lysates,
Lane 8: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified monoclonal antibody (Catalog # M00036) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91 kDa. The expected band size for STAT1 is at 91 kDa.
Protein Target Info & Infographic
Gene/Protein Information For STAT1 (Source: Uniprot.org, NCBI)
Gene Name
STAT1
Full Name
Signal transducer and activator of transcription 1-alpha/beta
Weight
91 kDa
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 1-alpha/beta;Transcription factor ISGF-3 components p91/p84;STAT1; STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT1, check out the STAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT1 alpha Rabbit Monoclonal Antibody (M00036)
Hello CJ!
M00036 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Synergistic inhibition of colon cancer growth by the combination of methylglyoxal and silencing of glyoxalase I mediated by the STAT1 pathway
Hepatitis C virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-alpha through dephosphorylation.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-STAT1 alpha Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-STAT1 alpha Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-STAT1 alpha Rabbit Monoclonal Antibody
Question
We have observed staining in human liver. Any tips? Is anti-STAT1 alpha Rabbit Monoclonal antibody supposed to stain liver positively?
B. Banerjee
Verified customer
Asked: 2020-04-16
Answer
Based on literature liver does express STAT1. Based on Uniprot.org, STAT1 is expressed in epithelium of bronchus, placenta testis, brain, b-cell, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-16
Question
I have attached the WB image, lot number and protocol we used for liver using anti-STAT1 alpha Rabbit Monoclonal antibody M00036. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-STAT1 alpha Rabbit Monoclonal antibody M00036 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The anti-STAT1 alpha Rabbit Monoclonal antibody (M00036) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-22
Question
I see that the anti-STAT1 alpha Rabbit Monoclonal antibody M00036 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-26
Question
Does anti-STAT1 alpha Rabbit Monoclonal antibody M00036 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
According to the expression profile of liver, STAT1 is highly expressed in liver. So, it is likely that anti-STAT1 alpha Rabbit Monoclonal antibody M00036 will work for WB with liver.
Boster Scientific Support
Answered: 2019-11-20
Question
We bought anti-STAT1 alpha Rabbit Monoclonal antibody for IP on cervix carcinoma erythroleukemia in the past. I am using human, and We want to use the antibody for WB next. We are interested in examining cervix carcinoma erythroleukemia as well as placenta testis in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
I viewed the website and datasheets of our anti-STAT1 alpha Rabbit Monoclonal antibody and it appears that M00036 has been tested on human in both IP and WB. Thus M00036 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-STAT1 alpha Rabbit Monoclonal antibody M00036. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-31
Question
I was wanting to use your anti-STAT1 alpha Rabbit Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
H. Jones
Verified customer
Asked: 2019-03-29
Answer
It shows on the product datasheet, M00036 anti-STAT1 alpha Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-29
Question
My question regards using your anti-STAT1 alpha Rabbit Monoclonal antibody for interleukin-6 signaling studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
We appreciate your inquiry. This M00036 anti-STAT1 alpha Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-27
Question
We were content with the WB result of your anti-STAT1 alpha Rabbit Monoclonal antibody. However we have observed positive staining in brain cytoplasm using this antibody. Is that expected? Could you tell me where is STAT1 supposed to be expressed?
H. Johnson
Verified customer
Asked: 2018-03-21
Answer
According to literature, brain does express STAT1. Generally STAT1 expresses in cytoplasm. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-03-21
Question
Is this M00036 anti-STAT1 alpha Rabbit Monoclonal antibody reactive to the isotypes of STAT1?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
The immunogen of M00036 anti-STAT1 alpha Rabbit Monoclonal antibody is A synthesized peptide derived from human STAT1 alpha . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-10
Question
Would M00036 anti-STAT1 alpha Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
It shows on the product datasheet, M00036 anti-STAT1 alpha Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-29
Question
We need to test anti-STAT1 alpha Rabbit Monoclonal antibody M00036 on human liver for research purposes, then I may be interested in using anti-STAT1 alpha Rabbit Monoclonal antibody M00036 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Thomas
Verified customer
Asked: 2016-03-04
Answer
The products we sell, including anti-STAT1 alpha Rabbit Monoclonal antibody M00036, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-03-04
