STAT1 Antibodies

Signal transducer and activator of transcription 1-alpha/beta (STAT1)

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. After type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases contributes to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.

The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to trigger the transcription of IFN-stimulated genes (ISG), which drive the mobile in an antiviral state.

Gene Information

Human
Gene Name:	STAT1
Uniprot:	P42224
Entrez:	6772

Family

Belongs to:
transcription factor STAT family

Alternative Names

CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91

Molecular Weight

Mass (kDA):
87.335 kDA

Genomic Context

Human
Location:	2q32.2
Sequence:	2; NC_000002.12 (190968989..191014250, complement)

Subcellular Localizations

Cytoplasm. Nucleus. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4 (PubMed:15322115). Monomethylation at Lys-525 is required for phosphorylation at Tyr-701 and translocation into the nucleus (PubMed:28753426). Translocates into the nucleus in response to interferon-beta stimulation (PubMed:26479788).

Diseases

• Neoplasms
• Malignant Neoplasms
• Inflammation
• Janiceps
• Virus Diseases
• Leukemia
• Carcinoma
• Infective Disorder
• Hepatitis
• Cell Transformation, Neoplastic
• Autoimmune Reaction
• Tissue Adhesions
• Liver Carcinoma

• Innate Immune Response
• Malignant Paraganglionic Neoplasm
• Hepatitis C
• Inflammatory Response
• Malignant Neoplasm Of Breast
• Melanoma
• Mammary Neoplasms

Interacting Proteins

• DDB1
• DDX58
• E2F1
• EGFR
• EIF1AD

• ERBB2
• FOS
• IFNAR1
• IFNAR2
• IFNGR1

• KDM3A
• MAVS
• OTUD4
• RXRA
• STAT2

• STAT3

Pathways

• Pathogenesis
• Immune Response
• Cell Proliferation
• Secretion
• Cell Growth
• Cell Death
• Localization
• Cell Cycle
• Inflammatory Response
• Cell Differentiation
• Cytokine Production
• Viral Replication
• Cell Activation

• Induction Of Apoptosis
• Methylation
• Innate Immune Response
• Angiogenesis
• Transport
• Translation
• Virulence

PTMs

• Phosphorylation
• Dephosphorylation
• Methylation
• Cleavage
• Acetylation
• Ubiquitination
• Sumoylation
• Nitration
• Reduction
• Demethylation

• Isgylation
• Glycosylation
• Deacetylation
• Biotinylation
• Oxidation
• Phosphorothioation
• Nitrosylation
• Prenylation
• Myristoylation
• Hydroxylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/STAT1

Boster Bio - Best Uses Of The STAT1 Marker

Boster Bio offers an Anti-STAT1 anti-microbial antibody which can be tested by using WB, ICC and IHC methods. We'll discuss the reliability of Boster Bio's STAT1 antibodies and the reasons you should look into purchasing one. We'll also give an description of the antibody's possible applications. In addition, we'll discuss the way Boster rewards first-time reviewers by providing product credits. This program is rewarding for scientists around the world and allows them access to high-quality antibodies.

Boster Bio Anti-STAT1 Antibody

It is essential to be able to react quickly to new ideas and guide the field in new directions for innovative research. Although antibodies that are cataloged are available but they are typically too expensive and inconvenient for pioneering research that involves rare organisms. GenScript provides custom antibody production services which allow researchers to obtain specific antibodies at a reasonable cost. These antibodies can make it possible to do cutting-edge research.

Boster Bio Validates Antibodies to WB, IHC

In 1993, the company was founded. Boster Bio has focused on making high-specific and sensitive ELISA kits and WB/IHC compatible antibodies. Their antibodies have been validated through testing them on 250 tissues, resulting in high affinity and specificity. Boster Bio's antibody has been cited in more than 29,000 scientific articles. To ensure their top quality and dependability, they provide a Boster Quality Guarantee.

Immunohistochemistry is based on the idea of antibodies binding to antigens. This method is extremely sensitive. Therefore, sample preparation for immunohistochemistry is vital. The Boster Bio protocol for immunohistochemistry provides detailed information about the preparation and analysis of samples, as along with a step by procedure overview of the process. Boster also offers a vast technical resource library , which includes blogs and information about diseases to assist researchers in maximizing the results of their experiments.

In addition to its high-quality products, Boster's strict quality standards extend to its reagents and kits for assays. Boster manufactures its antibodies and ELISA kits in-house and employs strict monitoring and optimizing the quality of every product. Boster also offers technical support for its products. It is a great choice for researchers who require top-quality antibodies but don't want compromise on quality.

WB is a suitable initial verification step. Monoclonal antibody are not able to function on proteins that have been denatured. This makes it difficult for WB results to be used for absolute evidence. IHC and flow cytometry are good methods to confirm the specificity of monoclonal antibodies. Negative controls are also crucial in these experiments as they assist in detecting nonspecific binding by secondary antibodies. This means that false positives will be identified.

Boster Bio Validates Antibodies for the ICC

For example, Boster Bio Anti-C-terminal-binding protein 2 (CTBP2) is a FITC conjugated ELISA antibody that reacts with Human. It is available in undiluted and diluted forms. It reacts with Human, Mouse and Rat. The FITC-conjugated antigen is available as a dilute solution and as a single agent reagent.

Boster Bio Validates Antibodies in IHC

Boster Bio's anti-STAT1 antigen is an phosphoY701-based fragment that has been tested for Reactivity in IP and WB. The antibody has been tested against human, mouse and rat tissues. Specific immunostaining protocols for each are included in the datasheet for the product. This product is recommended by the manufacturer to be used in IHC or IP applications.

It is important to verify the specificity of commercial anti-bodies. Cross-reactivity may occur when the antibody contains AMPK B2 proteins. This cross-reactivity should be documented in the datasheet. To ensure the specificity of the antibody the product must be used for IHC analysis and validation.

Subcellular localization and phospho-specificity are crucial criteria for IHC testing. In addition, phospho-specificity is a must. the antibodies must be evaluated for dilution and buffer efficiency. Validation is necessary before you can utilize for research. It is crucial to check the specificity of antibodies to ensure the security of your research. It is also possible to use negative or positive control cells in your research should you choose.

There are two options available to verify the validity of your antibody: IHC or STAT1 markers. The latter is more reliable however the former is more suitable for routine validation. Depending on the antibody's nature, you can select from a broad selection of secondary antibodies as well as isotype controls and detection systems. In this way, you are able to focus on the specific issues that require antibodies' confirmation.

To determine the specificity of an antibody you can test the specificity of an antibody using blocking peptides. These are the sequences used in the creation of the antibody. The antibodies are then incubated with these peptides to great quantity, staining the tissues that have the desired target. The antibody's ability to stain tissue is affected when the secondary antibody binds the peptide.

Boster Bio Validates Antibodies on WB

Boster Bio offers a wide variety of ELISA kits along with IHC and WB compatible antibody. The antibodies are validated for a wide range of applications that include IHC and WB. Boster Bio products are widely used and have been cited in more than 29,000 research papers. Find out more about how Boster Bio validates its antibodies. In this article, we'll describe how Boster Bio validates its antibodies using WB and IHC.

Boster's validation of antibodies includes testing samples that have been tested negative or positive. The tests are done to ensure that antibodies can react correctly with both positive and negative targets. Boster scientists are able report results for specific specimens, species, and applications. This process is available to scientists from all over the world. You must make sure that your antibodies are working on your specimens in order to get the best results.