Anti-SRP54 Antibody

Product Name

Anti-SRP54 Antibody

View all SRP54 Antibodies

SKU/Catalog Number

A06189

Size

100ul

Form

Liquid

Description

Boster Bio Anti-SRP54 Antibody catalog # A06189. Tested in WB,IHC,Flow Cytometry,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SRP54 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06189)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human StARD13

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06189 is reactive to SRP54 in Human, Mouse, Rat

Applications

A06189 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55705 MW

Background of SRP54

GTPase-activating protein for RhoA, and perhaps for Cdc42. May be involved in regulation of cytoskeletal reorganization, cell proliferation and cell motility. Acts a tumor suppressor in hepatocellular carcinoma cells.

Ching Y.-P., J. Biol. Chem. 278:10824-10830(2003).
Nagaraja G.M., Biochem. Biophys. Res. Commun. 313:654-665(2004).
Leung T.H.-Y., Proc. Natl. Acad. Sci. U.S.A. 102:15207-15212(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500
IP: 1:10-1:50
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of SRP54 using Anti-SRP54 Antibody (A06189).

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SRP54 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature

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Figure 1. Western blotting validation for Anti-SRP54 Antibody A06189

Western blot analysis of SRP54 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG -HRP Secondary Antibody (HA1001) at 1:5

Gene/Protein Information For SRP54 (Source: Uniprot.org, NCBI)

Alternative Names

signal recognition particle 54 kDa protein; signal recognition particle 54kD; signal recognition particle 54kDa SRP54 SCN8 signal recognition particle 54 signal recognition particle 54 kDa protein|signal recognition particle 54kD|signal recognition particle 54kDa

For more info on SRP54, check out the SRP54 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SRP54: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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