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Product Info Summary
| SKU: | A06189 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-SRP54 Antibody
SKU/Catalog Number
A06189
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SRP54 Antibody catalog # A06189. Tested in WB,IHC,Flow Cytometry,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SRP54 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06189)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein within human SRP54 aa 1-200.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06189 is reactive to SRP54 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
55705 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06189 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
IP: 1:10-1:50
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of SRP54 using Anti-SRP54 Antibody (A06189).
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SRP54 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature
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Figure 1. Western blotting validation for Anti-SRP54 Antibody A06189
Western blot analysis of SRP54 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG -HRP Secondary Antibody (HA1001) at 1:5Protein Target Info & Infographic
Gene/Protein Information For SRP54 (Source: Uniprot.org, NCBI)
Gene Name
SRP54
Full Name
Signal recognition particle 54 kDa protein
Weight
55705 MW
Superfamily
GTP-binding SRP family
Alternative Names
Signal recognition particle 54 kDa protein;SRP54;SRP54; SRP54 SCN8 signal recognition particle 54 signal recognition particle 54 kDa protein|signal recognition particle 54kD|signal recognition particle 54kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRP54, check out the SRP54 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRP54: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SRP54 Antibody (A06189)
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