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Product Info Summary
| SKU: | A06046 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-SR-1D HTR1D Antibody
SKU/Catalog Number
A06046
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SR-1D HTR1D Antibody catalog # A06046. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SR-1D HTR1D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06046)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human SR-1D. at AA range: 120-200
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06046 is reactive to HTR1D in Human, Mouse, Rat
Applications
A06046 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
41907 MW
Background of 5-HT1D
Cyclin T2 is a ubiquitously expressed nuclear protein that is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns that contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Anti-Cyclin T2 Antibody is useful for researchers interested in HIV research, Gene expression, and cell cycle pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HepG2 823 293T AD293 HeLa using SR-1D antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For HTR1D (Source: Uniprot.org, NCBI)
Gene Name
HTR1D
Full Name
5-hydroxytryptamine receptor 1D
Weight
41907 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
5HT1D; 5-HT1D; 5-HT-1D; 5-HT-1D-alpha; 5-HT1DHTR1DA; 5-hydroxytryptamine (serotonin) receptor 1D; HT1DA; HTR1D; HTR1DA; HTRL; RDC4; RDC4,5-hydroxytryptamine receptor 1D; Serotonin receptor 1D HTR1D 5-HT1D, HT1DAA, HTRL, RDC4, HTR1D 5-hydroxytryptamine receptor 1D 5-hydroxytryptamine receptor 1D|5-HT-1D|5-HT-1D-alpha|5-hydroxytryptamine (serotonin) receptor 1D, G protein-coupled|serotonin 1D alpha receptor|serotonin receptor 1D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HTR1D, check out the HTR1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HTR1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SR-1D HTR1D Antibody (A06046)
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No publications found for A06046
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Recommended Resources
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SR-1D HTR1D Antibody
Question
Will A06046 anti-SR-1D antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Lewis
Verified customer
Asked: 2019-09-26
Answer
As indicated on the product datasheet, A06046 anti-SR-1D antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-26
Question
I see that the anti-SR-1D antibody A06046 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-SR-1D antibody A06046. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-06
Question
We are currently using anti-SR-1D antibody A06046 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
The anti-SR-1D antibody (A06046) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-11
Question
I was wanting to use your anti-SR-1D antibody for WB for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?
J. Krishna
Verified customer
Asked: 2018-06-07
Answer
You can see on the product datasheet, A06046 anti-SR-1D antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-07
Question
Our lab want to know about to test anti-SR-1D antibody A06046 on mouse placenta for research purposes, then I may be interested in using anti-SR-1D antibody A06046 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Zhao
Verified customer
Asked: 2017-07-31
Answer
The products we sell, including anti-SR-1D antibody A06046, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-31
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-SR-1D antibody A06046. Please let me know if you require anything else.
A. Singh
Verified customer
Asked: 2015-12-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-14
