Anti-Sprouty-2 SPRY2 Antibody

Product Name

Anti-Sprouty-2 SPRY2 Antibody

View all SPRY2 Antibodies

SKU/Catalog Number

A02089-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Sprouty-2 SPRY2 Antibody (Catalog # A02089-1). Tested in ELISA, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Sprouty-2 SPRY2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02089-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-Sprouty-2 affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with peptide corresponding to an internal region of human sprouty-2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02089-1 is reactive to SPRY2 in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Observed Molecular Weight

42 kDa

Calculated molecular weight

98809 MW

Background of SPRY2

Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty-2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C-terminus of the protein. Significant sequence divergence occurs at the N-terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02089-1 is guaranteed for ELISA, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: User optimized
IHC: 10 µg/mL
IF Microscopy: User optimized
WB: 1:500-1:5,000

Validation Images & Assay Conditions

Gene/Protein Information For SPRY2 (Source: Uniprot.org, NCBI)

Alternative Names

Sprouty2, Sprouty-2, Sprouty 2, SPRY2, SPRY 2, Spry-2, Protein sprouty homolog 2 SPRY2 IGAN3, hSPRY2 sprouty RTK signaling antagonist 2 protein sprouty homolog 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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