Product Info Summary
SKU: | M02089 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Sprouty 2 SPRY2 Monoclonal Antibody
SKU/Catalog Number
M02089
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Sprouty 2 SPRY2 Monoclonal Antibody catalog # M02089. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sprouty 2 SPRY2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02089)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFOH-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Sprouty 2 May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02089 is reactive to SPRY2 in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
38 kDa
Calculated molecular weight
32583 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02089 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Sprouty 2 expression in HeLa cell lysate.
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SPRY2 (Source: Uniprot.org, NCBI)
Gene Name
SPRY2
Full Name
Protein sprouty homolog 2
Weight
32583 MW
Superfamily
sprouty family
Alternative Names
Junctional adhesion molecule A;JAM-A;Junctional adhesion molecule 1;JAM-1;Platelet F11 receptor;Platelet adhesion molecule 1;PAM-1;CD321;F11R;JAM1, JCAM;UNQ264/PRO301; SPRY2 IGAN3, hSPRY2 sprouty RTK signaling antagonist 2 protein sprouty homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPRY2, check out the SPRY2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPRY2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sprouty 2 SPRY2 Monoclonal Antibody (M02089)
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No publications found for M02089
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Customer Q&As
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1 Customer Q&As for Anti-Sprouty 2 SPRY2 Monoclonal Antibody
Question
We are currently using anti-Sprouty 2 Monoclonal antibody M02089 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The anti-Sprouty 2 Monoclonal antibody (M02089) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-27