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Product Info Summary
| SKU: | A10990 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SF3B14 SF3B6 Antibody
SKU/Catalog Number
A10990
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SF3B14 SF3B6 Antibody (Catalog # A10990). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SF3B14 SF3B6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10990)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human SF3B14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10990 is reactive to SF3B6 in Human, Mouse
Reconstitution
Calculated molecular weight
14585 MW
Background of SF3B6
Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
Will C.L., EMBO J. 20:4536-4546(2001).
Lai C.-H., Genome Res. 10:703-713(2000).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10990 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, using SF3B14 antibody A10990.
Protein Target Info & Infographic
Gene/Protein Information For SF3B6 (Source: Uniprot.org, NCBI)
Gene Name
SF3B6
Full Name
Splicing factor 3B subunit 6
Weight
14585 MW
Superfamily
SF3B6 family
Alternative Names
Splicing factor 3B subunit 6;Pre-mRNA branch site protein p14;SF3b 14 kDa subunit;SF3B14a;Spliceosome-associated protein, 14-kDa;Splicing factor 3b, subunit 6, 14kDa;SF3B6;SAP14, SF3B14, SF3B14A;CGI-110, HSPC175, HT006; SF3B6 CGI-110, HSPC175, Ht006, P14, SAP14, SAP14a, SF3B14, SF3B14a splicing factor 3b subunit 6 splicing factor 3B subunit 6|SF3b 14 kDa subunit|pre-mRNA branch site protein p14|spliceosome-associated protein, 14 kDa subunit|spliceosome-associated protein, 14-kDa|splicing factor 3B, 14 kDa subunit|splicing factor 3b, subunit 6, 14kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SF3B6, check out the SF3B6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SF3B6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SF3B14 SF3B6 Antibody (A10990)
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Customer Q&As
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4 Customer Q&As for Anti-SF3B14 SF3B6 Antibody
Question
My question regarding product A10990, anti-SF3B14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Mitchell
Verified customer
Asked: 2018-03-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10990 anti-SF3B14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-23
Question
We want to test anti-SF3B14 antibody A10990 on human testis for research purposes, then I may be interested in using anti-SF3B14 antibody A10990 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
The products we sell, including anti-SF3B14 antibody A10990, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-30
Question
I was wanting to use your anti-SF3B14 antibody for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
H. Collins
Verified customer
Asked: 2015-04-13
Answer
It shows on the product datasheet, A10990 anti-SF3B14 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-04-13
Question
I see that the anti-SF3B14 antibody A10990 works with WB, what is the protocol used to produce the result images on the product page?
K. Zhao
Verified customer
Asked: 2014-08-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-08-27
