Anti-Spondin-1 SPON1 Antibody

Product Name

Anti-Spondin-1 SPON1 Antibody

View all F-Spondin/SPON1 Antibodies

SKU/Catalog Number

A03094

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Spondin-1 SPON1 Antibody catalog # A03094. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Spondin-1 SPON1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03094)

Host

Rabbit

Contents

Liquid form in PBS containing 50% glycerol,and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human SPON1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03094 is reactive to SPON1 in Human, Mouse, Rat

Applications

A03094 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

90973 MW

Background of F-Spondin/SPON1

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.

McGuinness B.E., PLoS Biol. 3:433-449(2005).
Kitajima T.S., Curr. Biol. 15:353-359(2005).
Tang Z., Proc. Natl. Acad. Sci. U.S.A. 101:18012-18017(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-2000 ELISA 1:5000-20000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For SPON1 (Source: Uniprot.org, NCBI)

Alternative Names

FSpondin; F-Spondin; KIAA0762VSGP/F-spondin; MGC10724; SPON1; spondin 1, (f-spondin) extracellular matrix protein; spondin 1, extracellular matrix protein; spondin-1; Vascular smooth muscle cell growth-promoting factor; VSGP SPON1 VSGP/F-spondin, f-spondin spondin 1 spondin-1|spondin 1, (f-spondin) extracellular matrix protein|spondin 1, extracellular matrix protein|vascular smooth muscle cell growth-promoting factor

For more info on SPON1, check out the SPON1 Infographic

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In this infographic, you will see the following information for SPON1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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