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Product Info Summary
| SKU: | A05115 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Rab 25 Monoclonal Antibody
SKU/Catalog Number
A05115
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rab 25 Monoclonal Antibody catalog # A05115. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab 25 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05115)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3F12
Isotype
IgG1
Immunogen
Purified recombinant fragment of Rab 25 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05115 is reactive to RAB25 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
23496 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05115 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000
FC, 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using Rab 25 Monoclonal antibody against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
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Immunofluorescence (IF) analysis of A549 cells using Rab 25 Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Immunohistochemistry (IHC) analysis of paraffin-embedded esophagus tissues (left) and Human Lung Cancer (right) with DAB staining using Rab 25 Monoclonal antibody.
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Flow cytometric (FCM) analysis of NIH/3T3 cells using Rab 25 Monoclonal antibody (green) and negative control (purple).
Protein Target Info & Infographic
Gene/Protein Information For RAB25 (Source: Uniprot.org, NCBI)
Gene Name
RAB25
Full Name
Ras-related protein Rab-25
Weight
23496 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-25;CATX-8;RAB25 ;CATX8; Rab25|AV012978|RAB25, member RAS oncogene family|ras-related protein Rab-25
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB25, check out the RAB25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab 25 Monoclonal Antibody (A05115)
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No publications found for A05115
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Customer Q&As
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4 Customer Q&As for Anti-Rab 25 Monoclonal Antibody
Question
Can you help my question with product A05115, anti-Rab 25 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05115 anti-Rab 25 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-03
Question
Is this A05115 anti-Rab 25 Monoclonal antibody reactive to the isotypes of RAB25?
J. Banerjee
Verified customer
Asked: 2020-02-04
Answer
The immunogen of A05115 anti-Rab 25 Monoclonal antibody is Purified recombinant fragment of Rab 25 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus mucosa using anti-Rab 25 Monoclonal antibody A05115. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-23
Question
We are currently using anti-Rab 25 Monoclonal antibody A05115 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
The anti-Rab 25 Monoclonal antibody (A05115) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-01
