Anti-SPHK1 Antibody Picoband®

Sphingosine Kinase 1/SPHK1 antibody

Boster Bio Anti-SPHK1 Antibody Picoband® catalog # A01390-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A01390-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-SPHK1 Antibody Picoband®

View all Sphingosine Kinase 1/SPHK1 Antibodies

SKU/Catalog Number

A01390-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SPHK1 Antibody Picoband® catalog # A01390-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SPHK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01390-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SPHK1 recombinant protein (Position: R16-H267).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01390-1 is reactive to SPHK1 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

45-50 kDa

Calculated molecular weight

102380 MW

Background of Sphingosine Kinase 1/SPHK1

SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01390-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Positive Control

WB: human HepG2 whole cell, human MCF-7 whole cell, human A431 whole cell, human PC-3 whole cell, human Hela whole cell, human U20S whole cell, human Caco-2 whole cell, rat liver tissue, rat kidney tissue, rat RH35 whole cell, mouse liver tissue, mouse kidney tissue, mouse Neuro-2a whole cell
IHC: human hashintoto's thyroditis tissue, human papillary thyroid carcinoma tissue, human placenta tissue, human gall bladder adenosquamous carcinoma tissue, human gastric cancer tissue, human laryngeal squamous cell carcinoma tissue, human liver cancer tissue, human lung cancer tissue, rat brain tissue, rat lung tissue, mouse brain tissue
ICC/IF: HepG2 cell
FCM: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For SPHK1 (Source: Uniprot.org, NCBI)

Gene Name

SPHK1

Full Name

Sphingosine kinase 1

Weight

102380 MW

Alternative Names

Hexokinase-2;2.7.1.1;Hexokinase type II;HK II;Muscle form hexokinase;HK2; SPHK1 SPHK sphingosine kinase 1 sphingosine kinase 1|SK 1|SPK 1|acetyltransferase SPHK1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SPHK1, check out the SPHK1 Infographic

SPHK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SPHK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A01390-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-SPHK1 Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-SPHK1 Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Anti-SPHK1 Antibody Picoband®

Order DetailsPrice
A01390-1

100μg

$370
A01390-1-10ug

10μg sample (liquid)

$99
A01390-1-Biotin

100 μg Biotin conjugated

$570
A01390-1-Cy3

100 μg Cy3 conjugated

$570
A01390-1-Dylight488

100 μg Dylight488 conjugated

$570
A01390-1-Dylight550

100 μg Dylight550 conjugated

$570
A01390-1-Dylight594

100 μg Dylight594 conjugated

$570
A01390-1-FITC

100 μg FITC conjugated

$570
A01390-1-HRP

100 μg HRP conjugated

$570
A01390-1-APC

100 μg APC conjugated

$670
A01390-1-PE

100 μg PE conjugated

$670
A01390-1-iFluor647

100 μg iFluor647 conjugated

$670
A01390-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A01390-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.