Anti-Sp5 Antibody Picoband®

Sp5 antibody

Boster Bio Anti-Sp5 Antibody Picoband® catalog # PB9443. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9443
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Sp5 Antibody Picoband®

View all Sp5 Antibodies

SKU/Catalog Number

PB9443

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Sp5 Antibody Picoband® catalog # PB9443. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Sp5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9443)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Sp5, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9443 is reactive to SP5 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

42 kDa

Calculated molecular weight

41964 MW

Background of Sp5

Sp5 is mapped to 2q31.1. It is a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Elevated expression of Sp5 has been noted in several human tumors including hepatocellular carcinoma, gastric cancer and colon cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9443 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: MCF-7 Whole Cell
IHC: human placenta tissue

Validation Images & Assay Conditions

Gene/Protein Information For SP5 (Source: Uniprot.org, NCBI)

Gene Name

SP5

Full Name

Transcription factor Sp5

Weight

41964 MW

Superfamily

Sp1 C2H2-type zinc-finger protein family

Alternative Names

Transcription factor Sp5;SP5; Sp5|trans-acting transcription factor 5|transcription factor Sp5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SP5, check out the SP5 Infographic

SP5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Sp5 Antibody Picoband®

Question

Do you have a BSA free version of anti-Sp5 antibody PB9443 available?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Sp5 antibody PB9443 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-27

Question

We are currently using anti-Sp5 antibody PB9443 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

The anti-Sp5 antibody (PB9443) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-12

Question

My question regarding product PB9443, anti-Sp5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-12-17

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9443 anti-Sp5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-17

Question

I was wanting to use your anti-Sp5 antibody for WB for human endocervix on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human endocervix identification?

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

As indicated on the product datasheet, PB9443 anti-Sp5 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human endocervix in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-24

Order DetailsPrice
PB9443

100μg

$370
PB9443-10ug

10μg sample (liquid)

$99
PB9443-Biotin

100 μg Biotin conjugated

$570
PB9443-Cy3

100 μg Cy3 conjugated

$570
PB9443-Dylight488

100 μg Dylight488 conjugated

$570
PB9443-Dylight550

100 μg Dylight550 conjugated

$570
PB9443-Dylight594

100 μg Dylight594 conjugated

$570
PB9443-FITC

100 μg FITC conjugated

$570
PB9443-HRP

100 μg HRP conjugated

$570
PB9443-APC

100 μg APC conjugated

$670
PB9443-PE

100 μg PE conjugated

$670
PB9443-iFluor647

100 μg iFluor647 conjugated

$670
PB9443-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9443
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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