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Product Info Summary
| SKU: | M08724 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SOX1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M08724
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SOX1 Rabbit Monoclonal Antibody catalog # M08724. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08724)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GIA-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SOX1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08724 is reactive to SOX1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
39 kDa
Calculated molecular weight
39023 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08724 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human K562 whole cell, human Jurkat whole cell, human Caco-2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SOX1 using anti-SOX1 antibody (M08724).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SOX1 antigen affinity purified monoclonal antibody (Catalog # M08724) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX1 at approximately 39 kDa. The expected band size for SOX1 is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SOX1 (Source: Uniprot.org, NCBI)
Gene Name
SOX1
Full Name
Transcription factor SOX-1
Weight
39023 MW
Alternative Names
Transcription factor SOX-1;SOX1; SOX1 SRY-box transcription factor 1 transcription factor SOX-1|SRY (sex determining region Y)-box 1|SRY-box 1|SRY-related HMG-box gene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX1, check out the SOX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX1 Rabbit Monoclonal Antibody (M08724)
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No publications found for M08724
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Customer Q&As
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14 Customer Q&As for Anti-SOX1 Rabbit Monoclonal Antibody
Question
My lab would like using your anti-SOX1 Rabbit Monoclonal antibody for ventral spinal cord interneuron specification studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
I appreciate your inquiry. This M08724 anti-SOX1 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-12
Question
Is this M08724 anti-SOX1 Rabbit Monoclonal antibody reactive to the isotypes of SOX1?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The immunogen of M08724 anti-SOX1 Rabbit Monoclonal antibody is A synthesized peptide derived from human SOX1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-15
Question
I was wanting to use to test anti-SOX1 Rabbit Monoclonal antibody M08724 on mouse forebrain for research purposes, then I may be interested in using anti-SOX1 Rabbit Monoclonal antibody M08724 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The products we sell, including anti-SOX1 Rabbit Monoclonal antibody M08724, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-13
Question
We are currently using anti-SOX1 Rabbit Monoclonal antibody M08724 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
S. Jones
Verified customer
Asked: 2019-09-16
Answer
The anti-SOX1 Rabbit Monoclonal antibody (M08724) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-16
Question
Would M08724 anti-SOX1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
You can see on the product datasheet, M08724 anti-SOX1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-SOX1 Rabbit Monoclonal antibody M08724. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-30
Question
I was wanting to use your anti-SOX1 Rabbit Monoclonal antibody for IF for mouse forebrain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse forebrain identification?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
As indicated on the product datasheet, M08724 anti-SOX1 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse forebrain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-25
Question
Would anti-SOX1 Rabbit Monoclonal antibody M08724 work on primate IF with forebrain?
Verified Customer
Verified customer
Asked: 2017-08-14
Answer
Our lab technicians have not tested anti-SOX1 Rabbit Monoclonal antibody M08724 on primate. You can run a BLAST between primate and the immunogen sequence of anti-SOX1 Rabbit Monoclonal antibody M08724 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate forebrain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-14
Question
Do you have a BSA free version of anti-SOX1 Rabbit Monoclonal antibody M08724 available?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SOX1 Rabbit Monoclonal antibody M08724 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-09
Question
Can you help my question with product M08724, anti-SOX1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08724 anti-SOX1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-26
Question
Is a blocking peptide available for product anti-SOX1 Rabbit Monoclonal antibody (M08724)?
B. Krishna
Verified customer
Asked: 2017-04-27
Answer
We do provide the blocking peptide for product anti-SOX1 Rabbit Monoclonal antibody (M08724). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-04-27
Question
Here is the WB image, lot number and protocol we used for forebrain using anti-SOX1 Rabbit Monoclonal antibody M08724. Please let me know if you require anything else.
S. Anderson
Verified customer
Asked: 2016-03-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-14
Question
Would anti-SOX1 Rabbit Monoclonal antibody M08724 work for IF with forebrain?
P. Parker
Verified customer
Asked: 2014-09-26
Answer
According to the expression profile of forebrain, SOX1 is highly expressed in forebrain. So, it is likely that anti-SOX1 Rabbit Monoclonal antibody M08724 will work for IF with forebrain.
Boster Scientific Support
Answered: 2014-09-26
Question
I see that the anti-SOX1 Rabbit Monoclonal antibody M08724 works with IF, what is the protocol used to produce the result images on the product page?
R. Mangal
Verified customer
Asked: 2014-07-17
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-07-17
