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Product Info Summary
| SKU: | A05321 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BRSK2 Antibody
SKU/Catalog Number
A05321
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BRSK2 Antibody (Catalog # A05321). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
BRSK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BRSK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05321)
Host
Rabbit
Contents
BRSK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BRSK2 antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human BRSK2. The immunogen is located within amino acids 630 - 680 of BRSK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members.
Reactive Species
A05321 is reactive to BRSK2 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
81633 MW
Background of BRSK2
BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK2 is expressed in the brain and to a lesser extent in the testes. BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1. More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis. At least four isoforms of BRSK2 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05321 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BRSK2 antibody can be used for detection of BRSK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5
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Immunohistochemistry of BRSK2 in human brain tissue with BRSK2 antibody at 5 μg/mL.
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Immunofluorescence of BRSK2 in Human Brain cells with BRSK2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BRSK2 (Source: Uniprot.org, NCBI)
Gene Name
BRSK2
Full Name
Serine/threonine-protein kinase BRSK2
Weight
81633 MW
Superfamily
protein kinase superfamily
Alternative Names
SAD1, SADA, STK29, PEN11B, C11orf7, HUSSY-12, Brain-selective kinase 2 BRSK2 C11orf7, PEN11B, SAD1, SADA, STK29 BR serine/threonine kinase 2 serine/threonine-protein kinase BRSK2|BR serine/threonine-protein kinase 2|brain-selective kinase 2|brain-specific serine/threonine-protein kinase 2|protein kinase SAD1B|serine/threonine-protein kinase 29|serine/threonine-protein kinase SAD-A|testicular tissue protein Li 30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRSK2, check out the BRSK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRSK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRSK2 Antibody (A05321)
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