Click image to see more details
Product Info Summary
| SKU: | PB9403 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SOX1 Antibody Picoband®
SKU/Catalog Number
PB9403
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SOX1 Antibody Picoband® catalog # PB9403. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SOX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9403)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of Human SOX1, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9403 is reactive to SOX1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39023 MW
Background of SOX1
SOX1 (for Sex determining region Y-box 1), located in 13q34, is a transcription factor in the Sox protein family. It means that this intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development. SOX1 expression is restricted to neuroectodermin the tetrapod embryo. It is expressed particularly in the ventral striatum.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9403 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Brain Tissue, NIH3T3 Whole Cell
Validation Images & Assay Conditions
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Anti-SOX1 Picoband antibody, PB9403, Western blotting
All lanes: Anti SOX1 (PB9403) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
Protein Target Info & Infographic
Gene/Protein Information For SOX1 (Source: Uniprot.org, NCBI)
Gene Name
SOX1
Full Name
Transcription factor SOX-1
Weight
39023 MW
Alternative Names
Transcription factor SOX-1;SOX1; SOX1 SRY-box transcription factor 1 transcription factor SOX-1|SRY (sex determining region Y)-box 1|SRY-box 1|SRY-related HMG-box gene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX1, check out the SOX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SOX1 Antibody Picoband® (PB9403)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-SOX1 Antibody Picoband®
Question
Does PB9403 anti-SOX1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
You can see on the product datasheet, PB9403 anti-SOX1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-16
Question
I have attached the WB image, lot number and protocol we used for forebrain using anti-SOX1 antibody PB9403. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-SOX1 antibody PB9403. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-21
Question
Is a blocking peptide available for product anti-SOX1 antibody (PB9403)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We do provide the blocking peptide for product anti-SOX1 antibody (PB9403). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-19
Question
My question regarding product PB9403, anti-SOX1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9403 anti-SOX1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
Is this PB9403 anti-SOX1 antibody reactive to the isotypes of SOX1?
N. Jones
Verified customer
Asked: 2019-11-05
Answer
The immunogen of PB9403 anti-SOX1 antibody is A synthetic peptide corresponding to a sequence in the middle region of Human SOX1(215-248aa QHPGAGGAHPHAHPAHPHPHHPHAHPHNPQPMHR), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-05
Question
I see that the anti-SOX1 antibody PB9403 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-19
Question
I would like using your anti-SOX1 antibody for ventral spinal cord interneuron specification studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Thanks for your inquiry. This PB9403 anti-SOX1 antibody is validated on rat brain tissue, tissue lysate, nih3t3 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-01
Question
Do you have a BSA free version of anti-SOX1 antibody PB9403 available?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SOX1 antibody PB9403 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-18
Question
We are interested in to test anti-SOX1 antibody PB9403 on rat forebrain for research purposes, then I may be interested in using anti-SOX1 antibody PB9403 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The products we sell, including anti-SOX1 antibody PB9403, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-14
Question
I was wanting to use your anti-SOX1 antibody for WB for rat forebrain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat forebrain identification?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
As indicated on the product datasheet, PB9403 anti-SOX1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat forebrain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-09
Question
Would anti-SOX1 antibody PB9403 work for WB with forebrain?
Verified Customer
Verified customer
Asked: 2018-09-11
Answer
According to the expression profile of forebrain, SOX1 is highly expressed in forebrain. So, it is likely that anti-SOX1 antibody PB9403 will work for WB with forebrain.
Boster Scientific Support
Answered: 2018-09-11
Question
We are currently using anti-SOX1 antibody PB9403 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
The anti-SOX1 antibody (PB9403) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-12
