Product Info Summary
SKU: | PA2074 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ALDH3A1 Antibody
View all Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibodies
SKU/Catalog Number
PA2074
BA3033 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ALDH3A1 Antibody catalog # PA2074. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALDH3A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2074)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ALDH3A1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2074 is reactive to ALDH3A1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
50395 MW
Background of Aldehyde Dehydrogenase 3-A1/ALDH3A1
ALDH3A1 (Aldehyde Dehydrogenase, Family 3, Subfamily A, Member 1), also known as ALDH3, is an enzyme that in humans is encoded by the ALDH3A1 gene. By in situ hybridization, Hiraoka et al. (1995) mapped the ALDH3 gene to 17p11.2. ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2074 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human A549 whole cell, human Hela whole cell
IHC: human rectal cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (PA2074).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # PA2074) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 50 kDa. The expected band size for ALDH3A1 is at 50 kDa.
Click image to see more details
Figure 2. IHC analysis of ALDH3A1 using anti-ALDH3A1 antibody (PA2074).
ALDH3A1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH3A1 Antibody (PA2074) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ALDH3A1 (Source: Uniprot.org, NCBI)
Gene Name
ALDH3A1
Full Name
Aldehyde dehydrogenase, dimeric NADP-preferring
Weight
50395 MW
Superfamily
aldehyde dehydrogenase family
Alternative Names
Aldehyde dehydrogenase, dimeric NADP-preferring;1.2.1.5;ALDHIII;Aldehyde dehydrogenase 3;Aldehyde dehydrogenase family 3 member A1;ALDH3A1;ALDH3; ALDH3A1 ALDH3, ALDHIII aldehyde dehydrogenase 3 family member A1 aldehyde dehydrogenase, dimeric NADP-preferring|aldehyde dehydrogenase isozyme 3|aldehyde dehydrogenase type III|stomach aldehyde dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALDH3A1, check out the ALDH3A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALDH3A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALDH3A1 Antibody (PA2074)
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Customer Q&As
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6 Customer Q&As for Anti-ALDH3A1 Antibody
Question
Does PA2074 anti-ALDH3A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
As indicated on the product datasheet, PA2074 anti-ALDH3A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-07
Question
We are currently using anti-ALDH3A1 antibody PA2074 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-ALDH3A1 antibody (PA2074) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix tongue using anti-ALDH3A1 antibody PA2074. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-11
Question
Is this PA2074 anti-ALDH3A1 antibody reactive to the isotypes of ALDH3A1?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
The immunogen of PA2074 anti-ALDH3A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ALDH3A1(440-453aa KVRYPPSPAKMTQH). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-04
Question
I was wanting to use your anti-ALDH3A1 antibody for WB for human cervix tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix tongue identification?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
You can see on the product datasheet, PA2074 anti-ALDH3A1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-31
Question
Will anti-ALDH3A1 antibody PA2074 work on dog IHC with nasal cavity epithelium?
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
Our lab technicians have not validated anti-ALDH3A1 antibody PA2074 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ALDH3A1 antibody PA2074 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog nasal cavity epithelium in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-27