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Product Info Summary
| SKU: | PA1939 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cullin 3/CUL3 Antibody Picoband®
SKU/Catalog Number
PA1939
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cullin 3/CUL3 Antibody catalog # PA1939. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cullin 3/CUL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1939)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Cullin 3, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1939 is reactive to CUL3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
89 kDa
Calculated molecular weight
88930 MW
Background of Cullin 3
Cullin-3 (Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al. (1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz (2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37). Sumara et al. (2007) found that KLHL9, KLHL13, and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates. Rondou et al. (2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4 (DRD4). KLHL12 interacted directly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al. (2011) showed that KLHL7 interacted with CUL3 and ROC1 (RBX1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1939 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human K562 whole cell, human Ramos whole cell, rat brain tissue, mouse brain tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cullin3 using anti-Cullin3 antibody (PA1939).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Ramos whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin3 antigen affinity purified polyclonal antibody (Catalog # PA1939) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin3 at approximately 89 kDa. The expected band size for Cullin3 is at 89 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CUL3 (Source: Uniprot.org, NCBI)
Gene Name
CUL3
Full Name
Cullin-3
Weight
88930 MW
Superfamily
cullin family
Alternative Names
Cullin-3;CUL-3;CUL3;KIAA0617; CUL3 CUL-3, PHA2E cullin 3 cullin-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CUL3, check out the CUL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CUL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cullin 3/CUL3 Antibody Picoband® (PA1939)
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No publications found for PA1939
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Customer Q&As
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1 Customer Q&As for Anti-Cullin 3/CUL3 Antibody Picoband®
Question
We are currently using anti-Cullin 3/CUL3 antibody PA1939 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
The anti-Cullin 3/CUL3 antibody (PA1939) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-07
