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Product Info Summary
| SKU: | M33970-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-SODM Antibody
View all SOD2/Mn-SOD Antibodies
SKU/Catalog Number
M33970-1
Size
50 µl
Description
Boster Bio Anti-SODM Antibody (Catalog # M33970-1). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SODM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33970-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1559CT572.3.83
Isotype
IgG1,κ
Immunogen
This SODM antibody is generated from a mouse immunized with a recombinant protein of human SODM.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M33970-1 is reactive to SOD2 in Human, Mouse
Applications
M33970-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
24750 Da
Background of SOD2/Mn-SOD
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-SODM Antibody at 1:2000 dilution
Lane 1: human brain lysate
Lane 2: Hela whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M33970-1 staining SODM in human skeletal muscle sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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M33970-1 staining SODM in human brain sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For SOD2 (Source: Uniprot.org, NCBI)
Gene Name
SOD2
Full Name
Superoxide dismutase [Mn], mitochondrial
Weight
24750 Da
Superfamily
iron/manganese superoxide dismutase family
Alternative Names
EC 1.15.1.1; indophenoloxidase B; IPOB; IPO-B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn SOD; Mn superoxide dismutase; MnSOD; MVCD6; SOD, Mitochodrial; SOD2; superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2, mitochondrial SOD2 GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD superoxide dismutase 2 superoxide dismutase [Mn], mitochondrial|Mn superoxide dismutase|epididymis secretory sperm binding protein|gastric cancer-associated lncRNA 1|indophenoloxidase B|manganese-containing superoxide dismutase|mangano-superoxide dismutase|superoxide dismutase 2, mitochondrial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOD2, check out the SOD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SODM Antibody (M33970-1)
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