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Product Info Summary
| SKU: | A00349 |
|---|---|
| Size: | 200µg |
| Reactive Species: | Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus, Clam, Porcine |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, IHC-P, WB |
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Product info
Product Name
Anti-Mn SOD Sod2 Antibody
View all SOD2/Mn-SOD Antibodies
SKU/Catalog Number
A00349
Size
200µg
Form
Liquid
Description
Boster Bio Anti-Mn SOD Sod2 Antibody (Catalog# A00349). Tested in ELISA, Flow Cytometry, IHC-P, IP, WB application(s). This antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Clam, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus.
Storage & Handling
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Cite This Product
Anti-Mn SOD Sod2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00349)
Host
Rabbit
Contents
Liquid. In sodium phosphate buffer, pH 7.0, containing 15mg/ml BSA, 0.09% sodium azide, and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Native rat Mn SOD
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00349 is reactive to Sod2 in Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus, Clam, Porcine
Reconstitution
Calculated molecular weight
83786 MW
Background of SOD2/Mn-SOD
Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00349 is guaranteed for ELISA, Flow Cytometry, IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1:100)
Immunoprecipitation (1:100)
Western Blot (1:1,000, colorimetric). Detects a band of ~25kDa.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Flow cytometry analysis of U251 cells using Mn SOD, pAb (right) and control rabbit IgG (left).
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Figure 1. Western blot analysis of Sod2 using anti-Sod2 antibody (A00349).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sod2 antigen affinity purified polyclonal antibody (Catalog # A00349) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Sod2.
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Figure 2. IHC analysis of Sod2 using anti-Sod2 antibody (A00349).
Sod2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Sod2 Antibody (A00349) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Sod2 (Source: Uniprot.org, NCBI)
Gene Name
Sod2
Full Name
Superoxide dismutase [Mn], mitochondrial
Weight
83786 MW
Superfamily
iron/manganese superoxide dismutase family
Alternative Names
muscle glycogen synthase pS641, glycogen synthase pS641, Glycogen, Glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen synthase1, GYS 1, GYS-1, GYS1, Starch synthase muscle SOD2 GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD superoxide dismutase 2 superoxide dismutase [Mn], mitochondrial|Mn superoxide dismutase|epididymis secretory sperm binding protein|gastric cancer-associated lncRNA 1|indophenoloxidase B|manganese-containing superoxide dismutase|mangano-superoxide dismutase|superoxide dismutase 2, mitochondrial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sod2, check out the Sod2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sod2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mn SOD Sod2 Antibody (A00349)
Hello CJ!
A00349 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation
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Customer Q&As
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16 Customer Q&As for Anti-Mn SOD Sod2 Antibody
Question
Our lab used your anti-Mn SOD antibody for Flow Cytometry on colon in a previous experiment. I am using rat, and We want to use the antibody for IHC next. We want examining colon as well as gastrocnemius in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
I looked at the website and datasheets of our anti-Mn SOD antibody and it seems that A00349 has been tested on rat in both Flow Cytometry and IHC. Thus A00349 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-23
Question
My colleagues were satisfied with the WB result of your anti-Mn SOD antibody. However we have seen positive staining in lung mitochondrion matrix. using this antibody. Is that expected? Could you tell me where is SOD2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
According to literature, lung does express SOD2. Generally SOD2 expresses in mitochondrion matrix. Regarding which tissues have SOD2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1988135, 7702755
Heart, Pubmed ID: 7498159, 7895732
Hippocampus, Testis, Tongue, and Uterus, Pubmed ID: 14702039
Liver, Pubmed ID: 2831093, 24275569
Lung, Pubmed ID: 15489334
Mammary carcinoma, Pubmed ID: 9150946
Boster Scientific Support
Answered: 2020-03-24
Question
My question regarding product A00349, anti-Mn SOD antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Banerjee
Verified customer
Asked: 2019-12-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00349 anti-Mn SOD antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-25
Question
Please see the WB image, lot number and protocol we used for lung using anti-Mn SOD antibody A00349. Please let me know if you require anything else.
S. Li
Verified customer
Asked: 2019-12-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
Is a blocking peptide available for product anti-Mn SOD antibody (A00349)?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We do provide the blocking peptide for product anti-Mn SOD antibody (A00349). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-07
Question
Do you have a BSA free version of anti-Mn SOD antibody A00349 available?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mn SOD antibody A00349 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-11
Question
I was wanting to use your anti-Mn SOD antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
It shows on the product datasheet, A00349 anti-Mn SOD antibody has been tested for ELISA, Flow Cytometry, IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Mn SOD antibody A00349. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-26
Question
We have observed staining in rat lung. Do you have any suggestions? Is anti-Mn SOD antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
From literature lung does express SOD2. From Uniprot.org, SOD2 is expressed in gastrocnemius, liver, colon, hippocampus, testis, tongue uterus, lung, heart, mammary carcinoma, among other tissues. Regarding which tissues have SOD2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1988135, 7702755
Heart, Pubmed ID: 7498159, 7895732
Hippocampus, Testis, Tongue, and Uterus, Pubmed ID: 14702039
Liver, Pubmed ID: 2831093, 24275569
Lung, Pubmed ID: 15489334
Mammary carcinoma, Pubmed ID: 9150946
Boster Scientific Support
Answered: 2019-01-09
Question
Would anti-Mn SOD antibody A00349 work for WB with lung?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
According to the expression profile of lung, SOD2 is highly expressed in lung. So, it is likely that anti-Mn SOD antibody A00349 will work for WB with lung.
Boster Scientific Support
Answered: 2018-05-18
Question
Our lab want to know about to test anti-Mn SOD antibody A00349 on human lung for research purposes, then I may be interested in using anti-Mn SOD antibody A00349 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
The products we sell, including anti-Mn SOD antibody A00349, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-19
Question
We are currently using anti-Mn SOD antibody A00349 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
The anti-Mn SOD antibody (A00349) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-12
Question
I see that the anti-Mn SOD antibody A00349 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-22
Question
Would A00349 anti-Mn SOD antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
As indicated on the product datasheet, A00349 anti-Mn SOD antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-09
Question
I was wanting to use using your anti-Mn SOD antibody for response to radiation studies. Has this antibody been tested with western blotting on u251 cells? We would like to see some validation images before ordering.
R. Williams
Verified customer
Asked: 2015-08-07
Answer
Thank you for your inquiry. This A00349 anti-Mn SOD antibody is tested on u251 cells. It is guaranteed to work for ELISA, Flow Cytometry, IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-08-07
Question
Is this A00349 anti-Mn SOD antibody reactive to the isotypes of SOD2?
C. Wu
Verified customer
Asked: 2013-03-12
Answer
The immunogen of A00349 anti-Mn SOD antibody is Native rat Mn SOD. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-12
