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Product Info Summary
| SKU: | A09010-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SNX18 Antibody Picoband®
SKU/Catalog Number
A09010-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SNX18 Antibody Picoband® catalog # A09010-2. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SNX18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09010-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SNX18 recombinant protein (Position: R22-F628).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09010-2 is reactive to SNX18 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
68.894kDa
Background of SNX18
Sorting nexin-18 is a protein that in humans is encoded by the SNX18 gene. This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a SH3 domain. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09010-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HL-60 whole cell, human MCF-7 whole cell, rat lung tisue, rat kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SNX18 using anti-SNX18 antibody (A09010-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat lung tisue lysates,
Lane 4: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX18 antigen affinity purified polyclonal antibody (Catalog # A09010-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX18 at approximately 69 kDa. The expected band size for SNX18 is at 69 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SNX18 (Source: Uniprot.org, NCBI)
Gene Name
SNX18
Full Name
Sorting nexin-18
Weight
68.894kDa
Superfamily
sorting nexin family
Alternative Names
Tudor domain-containing protein 3; TDRD3 SNX18 SH3PX2, SH3PXD3B, SNAG1 sorting nexin 18 sorting nexin-18|SH3 and PX domain-containing protein 3B|sorting nexin associated golgi protein 1|sorting nexin-associated Golgi protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNX18, check out the SNX18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNX18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SNX18 Antibody Picoband® (A09010-2)
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