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Product Info Summary
| SKU: | PA1814 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HSPA2 Antibody Picoband®
SKU/Catalog Number
PA1814
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSPA2 Antibody catalog # PA1814. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSPA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1814)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1814 is reactive to HSPA2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
70021 MW
Background of HSPA2
HSPA2 (heat shock 70kDa protein 2) also known as HEAT-SHOCK PROTEIN, 70-KD, 2, HSP70-2, HEAT-SHOCK PROTEIN, 70-KD, 3 or HSP70-3. Analysis of the sequence indicated that HSPA2 is the human homolog of the murine Hsp70-2 gene, with 91.7% identity in the nucleotide coding sequence and 98.2% in the corresponding amino acid sequence.HSPA2 has less amino acid homology to the other members of the human HSP70 gene family. HSPA2 is constitutively expressed in most tissues, with very high levels in testis and skeletal muscle. The HSPA2 gene is located on chromosome 14q22-q24. Immunohistochemical analysis detected weak expression of HSPA2 in spermatocytes and stronger expression in spermatids and in the tail of mature sperm. HSPA2 may be critical to sperm maturation through its role as a protein chaperone.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1814 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Testis Tissue, A549 Cell, MCF-7 Cell, HELA Cell, mouse spleen Tissue, mouse testis tissue,
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HSPA2 using anti-HSPA2 antibody (PA1814).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis Tissue Lysate,
Lane 2: A549 Cell Lysate,
Lane 3: MCF-7 Cell Lysate,
Lane 4: HELA Cell Lysate .
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 antigen affinity purified polyclonal antibody (Catalog # PA1814) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70KD. The expected band size for HSPA2 is at 70KD.
Click image to see more details
Figure 2. Western blot analysis of HSPA2 using anti-HSPA2 antibody (PA1814).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen Tissue Lysate,
Lane 2: mouse testis tissue Lysate,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 antigen affinity purified polyclonal antibody (Catalog # PA1814) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70KD. The expected band size for HSPA2 is at 70KD.
Protein Target Info & Infographic
Gene/Protein Information For HSPA2 (Source: Uniprot.org, NCBI)
Gene Name
HSPA2
Full Name
Heat shock-related 70 kDa protein 2
Weight
70021 MW
Superfamily
heat shock protein 70 family
Alternative Names
Heat shock-related 70 kDa protein 2;Heat shock 70 kDa protein 2;HSPA2; HSPA2 HSP70-2, HSP70-3 heat shock protein family A (Hsp70) member 2 heat shock-related 70 kDa protein 2|epididymis secretory sperm binding protein|heat shock 70kD protein 2|heat shock 70kDa protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA2, check out the HSPA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSPA2 Antibody Picoband® (PA1814)
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Customer Q&As
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5 Customer Q&As for Anti-HSPA2 Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain eye using anti-HSPA2 antibody PA1814. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
Is this PA1814 anti-HSPA2 antibody reactive to the isotypes of HSPA2?
D. Li
Verified customer
Asked: 2018-06-27
Answer
The immunogen of PA1814 anti-HSPA2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA2(580-599aa VINWLDRNQMAEKDEYEHKQ), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-27
Question
Is a blocking peptide available for product anti-HSPA2 antibody (PA1814)?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
We do provide the blocking peptide for product anti-HSPA2 antibody (PA1814). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-02
Question
Can you help my question with product PA1814, anti-HSPA2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Anderson
Verified customer
Asked: 2017-07-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1814 anti-HSPA2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-12
Question
I am looking for to test anti-HSPA2 antibody PA1814 on rat brain eye for research purposes, then I may be interested in using anti-HSPA2 antibody PA1814 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Walker
Verified customer
Asked: 2013-03-04
Answer
The products we sell, including anti-HSPA2 antibody PA1814, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-04
