Anti-SNAP23 Antibody Picoband®

Product Name

Anti-SNAP23 Antibody Picoband®

View all SNAP23 Antibodies

SKU/Catalog Number

PA1774

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SNAP23 Antibody catalog # PA1774. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SNAP23 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1774)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP23, different from the related rat and mouse sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1774 is reactive to SNAP23 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

105 kDa

Calculated molecular weight

23354 MW

Background of SNAP23

SNAP23 (Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene. The SNAP23 gene has 8 exons, with the initiation codon located in exon 2. The SNAP23 gene is mapped on 15q15.1-q15.2. The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1774 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Positive Control

WB: Rat Spleen Tissue, Rat testis Tissue, Rat Ovary Tissue, HELA Cell, MCF-7 Cell, SKOV Cell,
IHC: Human Placenta Tissue, human tonsil tissue
IHC-F: Human Placenta Tissue
ICC/IF: T47D cell
ICC: K562 Cell
FCM: HEPG2 cell

Validation Images & Assay Conditions

Gene/Protein Information For SNAP23 (Source: Uniprot.org, NCBI)

Alternative Names

Synaptosomal-associated protein 23;SNAP-23;Vesicle-membrane fusion protein SNAP-23;SNAP23; SNAP23 HsT17016, SNAP-23A, SNAP23B, SNAP23 synaptosome associated protein 23 synaptosomal-associated protein 23|synaptosomal-associated protein, 23kD|synaptosomal-associated protein, 23kDa|synaptosome associated protein 23kDa|vesicle-membrane fusion protein SNAP-23

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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