Anti-SMURF1 Antibody Picoband®

SMURF1 antibody

Boster Bio Anti-SMURF1 Antibody Picoband® catalog # PB9892. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9892
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-SMURF1 Antibody Picoband®

View all SMURF1 Antibodies

SKU/Catalog Number

PB9892

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SMURF1 Antibody Picoband® catalog # PB9892. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMURF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9892)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SMURF1, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9892 is reactive to SMURF1 in Human, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

86 kDa

Calculated molecular weight

86114 MW

Background of SMURF1

E3 ubiquitin-protein ligase SMURF1 is an enzyme that in humans is encoded by the SMURF1 gene. It is mapped to 7q22.1. This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9892 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: rat brain tissue, MCF-7 whole cell
IHC: human intestinal cancer tissue, human breast cancer tissue
ICC/IF: Hela cell
FCM: MCF-7 cell

Validation Images & Assay Conditions

Gene/Protein Information For SMURF1 (Source: Uniprot.org, NCBI)

Gene Name

SMURF1

Full Name

E3 ubiquitin-protein ligase SMURF1

Weight

86114 MW

Alternative Names

E3 ubiquitin-protein ligase SMURF1;hSMURF1;6.3.2.-;SMAD ubiquitination regulatory factor 1;SMAD-specific E3 ubiquitin-protein ligase 1;SMURF1;KIAA1625; SMURF1 SMAD specific E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase SMURF1|E3 ubiquitin ligase SMURF1|HECT-type E3 ubiquitin transferase SMURF1|Smad ubiquitination regulatory factor 1|Smad-specific E3 ubiquitin ligase 1|hSMURF1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SMURF1, check out the SMURF1 Infographic

SMURF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SMURF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-SMURF1 Antibody Picoband®

Question

We are currently using anti-SMURF1 antibody PB9892 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

The anti-SMURF1 antibody (PB9892) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-25

Order DetailsPrice
PB9892

100μg

$370
PB9892-10ug

10μg sample (liquid)

$99
PB9892-Biotin

100 μg Biotin conjugated

$570
PB9892-Cy3

100 μg Cy3 conjugated

$570
PB9892-Dylight488

100 μg Dylight488 conjugated

$570
PB9892-Dylight550

100 μg Dylight550 conjugated

$570
PB9892-Dylight594

100 μg Dylight594 conjugated

$570
PB9892-FITC

100 μg FITC conjugated

$570
PB9892-HRP

100 μg HRP conjugated

$570
PB9892-APC

100 μg APC conjugated

$670
PB9892-PE

100 μg PE conjugated

$670
PB9892-iFluor647

100 μg iFluor647 conjugated

$670
PB9892-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9892
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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