Anti-SMURF1 Antibody

Product Name

Anti-SMURF1 Antibody

View all SMURF1 Antibodies

SKU/Catalog Number

A02823

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SMURF1 Antibody (Catalog # A02823). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SMURF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-SMURF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02823)

Host

Rabbit

Contents

SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1. The immunogen is located within amino acids 180 - 230 of SMURF1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.

Reactive Species

A02823 is reactive to SMURF1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

86114 MW

Background of SMURF1

SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A02823 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For SMURF1 (Source: Uniprot.org, NCBI)

Alternative Names

SMAD specific E3 ubiquitin protein ligase 1, SMAD ubiquitination regulatory factor 1, E3 ubiquitin-protein ligase SMURF1 SMURF1 SMAD specific E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase SMURF1|E3 ubiquitin ligase SMURF1|HECT-type E3 ubiquitin transferase SMURF1|Smad ubiquitination regulatory factor 1|Smad-specific E3 ubiquitin ligase 1|hSMURF1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis