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Product Info Summary
| SKU: | M02585 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SMURF 2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02585
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SMURF 2 Rabbit Monoclonal Antibody catalog # M02585. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMURF 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02585)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AECF-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SMURF 2 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02585 is reactive to SMURF2 in Human, Mouse, Rat
Applications
M02585 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
41 kDa
Calculated molecular weight
209622 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of SMURF 2 expression in SH-SY-5Y cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For SMURF2 (Source: Uniprot.org, NCBI)
Gene Name
SMURF2
Full Name
E3 ubiquitin-protein ligase SMURF2
Weight
209622 MW
Alternative Names
DKFZp686F0270; E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; EC 6.3.2; EC 6.3.2.-; hSMURF2; MGC138150; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF2 SMURF2 SMAD specific E3 ubiquitin protein ligase 2 E3 ubiquitin-protein ligase SMURF2|E3 ubiquitin ligase SMURF2|HECT-type E3 ubiquitin transferase SMURF2|SMAD ubiquitination regulatory factor 2|hSMURF2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMURF2, check out the SMURF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMURF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMURF 2 Rabbit Monoclonal Antibody (M02585)
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No publications found for M02585
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SMURF 2 Rabbit Monoclonal Antibody
Question
What sample target on Western Blot application was tested with M02585?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of SMURF 2 expression in SH-SY-5Y cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
Would M02585 anti-SMURF 2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
As indicated on the product datasheet, M02585 anti-SMURF 2 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-25
Question
I was wanting to use to test anti-SMURF 2 Monoclonal antibody M02585 on rat brain for research purposes, then I may be interested in using anti-SMURF 2 Monoclonal antibody M02585 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Carter
Verified customer
Asked: 2018-09-24
Answer
The products we sell, including anti-SMURF 2 Monoclonal antibody M02585, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-24
Question
Is there a BSA free version of anti-SMURF 2 Monoclonal antibody M02585 available?
Verified Customer
Verified customer
Asked: 2018-02-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SMURF 2 Monoclonal antibody M02585 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SMURF 2 Monoclonal antibody M02585. Let me know if you need anything else.
B. Li
Verified customer
Asked: 2017-07-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-26
Question
Is this M02585 anti-SMURF 2 Monoclonal antibody reactive to the isotypes of SMURF2?
H. Carter
Verified customer
Asked: 2015-06-16
Answer
The immunogen of M02585 anti-SMURF 2 Monoclonal antibody is A synthesized peptide derived from human SMURF 2 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-06-16
Question
I have a question about product M02585, anti-SMURF 2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Gonzalez
Verified customer
Asked: 2014-12-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02585 anti-SMURF 2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-12
