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Product Info Summary
| SKU: | A08337 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SMIT SLC5A3 Antibody
SKU/Catalog Number
A08337
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SMIT SLC5A3 Antibody catalog # A08337. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMIT SLC5A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08337)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SLC5A3. AA range:221-270
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08337 is reactive to SLC5A3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
79694 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08337 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of HuvEc cells using SMIT Polyclonal Antibody diluted at 1：500
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Western blot analysis of various cells using SMIT Polyclonal Antibody diluted at 1：500
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Western blot analysis of lysates from HUVEC cells, using SLC5A3 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SLC5A3 (Source: Uniprot.org, NCBI)
Gene Name
SLC5A3
Full Name
Sodium/myo-inositol cotransporter
Weight
79694 MW
Superfamily
sodium:solute symporter (SSF) (TC 2.A.21) family
Alternative Names
Sodium/myo-inositol cotransporter;Na (+)/myo-inositol cotransporter;Sodium/myo-inositol transporter 1;SMIT1;Solute carrier family 5 member 3;SLC5A3; SLC5A3 BCW2, SMIT, SMIT1, SMIT2 solute carrier family 5 member 3 sodium/myo-inositol cotransporter|Na(+)/myo-inositol cotransporter|sodium/myo-inositol cotransporter 1|sodium/myo-inositol transporter 1|solute carrier family 5 (inositol transporters), member 3|solute carrier family 5 (sodium/myo-inositol cotransporter), member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC5A3, check out the SLC5A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC5A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMIT SLC5A3 Antibody (A08337)
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No publications found for A08337
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SMIT SLC5A3 Antibody
Question
I was wanting to use to test anti-SMIT antibody A08337 on human placenta for research purposes, then I may be interested in using anti-SMIT antibody A08337 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The products we sell, including anti-SMIT antibody A08337, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-10
Question
Does anti-SMIT antibody A08337 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
According to the expression profile of placenta, SLC5A3 is highly expressed in placenta. So, it is likely that anti-SMIT antibody A08337 will work for WB with placenta.
Boster Scientific Support
Answered: 2020-03-02
Question
We are currently using anti-SMIT antibody A08337 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The anti-SMIT antibody (A08337) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-27
Question
I see that the anti-SMIT antibody A08337 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-07
Question
Is there a BSA free version of anti-SMIT antibody A08337 available?
M. Wu
Verified customer
Asked: 2019-05-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SMIT antibody A08337 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-17
Question
Would A08337 anti-SMIT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
You can see on the product datasheet, A08337 anti-SMIT antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-22
Question
I was wanting to use your anti-SMIT antibody for WB for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
It shows on the product datasheet, A08337 anti-SMIT antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-11
