Click image to see more details
Product Info Summary
| SKU: | M00074 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Smad4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00074
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Smad4 Rabbit Monoclonal Antibody catalog # M00074. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00074)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FH-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00074 is reactive to SMAD4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
60439 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00074 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human breast cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SMAD4 expression in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Smad4 Antibody.
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SMAD4 (Source: Uniprot.org, NCBI)
Gene Name
SMAD4
Full Name
Mothers against decapentaplegic homolog 4
Weight
60439 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;Deletion target in pancreatic carcinoma 4;SMAD family member 4;SMAD 4;Smad4;hSMAD4;SMAD4;DPC4, MADH4; SMAD4 DPC4, JIP, MADH4, MYHRS SMAD family member 4 mothers against decapentaplegic homolog 4|MAD homolog 4|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|deletion target in pancreatic carcinoma 4|mothers against decapentaplegic, Drosophila, homolog of, 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD4, check out the SMAD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad4 Rabbit Monoclonal Antibody (M00074)
Hello CJ!
M00074 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
TGF%u2010%u03B2 regulates the proliferation of lung adenocarcinoma cells by inhibiting PIK3R3 expression
Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Smad4 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Smad4 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Smad4 Rabbit Monoclonal Antibody
Question
I am looking for to test anti-Smad4 Rabbit Monoclonal antibody M00074 on human kidney for research purposes, then I may be interested in using anti-Smad4 Rabbit Monoclonal antibody M00074 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The products we sell, including anti-Smad4 Rabbit Monoclonal antibody M00074, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-07
Question
Is a blocking peptide available for product anti-Smad4 Rabbit Monoclonal antibody (M00074)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We do provide the blocking peptide for product anti-Smad4 Rabbit Monoclonal antibody (M00074). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-28
Question
We are currently using anti-Smad4 Rabbit Monoclonal antibody M00074 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The anti-Smad4 Rabbit Monoclonal antibody (M00074) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Smad4 Rabbit Monoclonal antibody M00074. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-13
Question
Will M00074 anti-Smad4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
As indicated on the product datasheet, M00074 anti-Smad4 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-21
Question
I see that the anti-Smad4 Rabbit Monoclonal antibody M00074 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-19
Question
Do you have a BSA free version of anti-Smad4 Rabbit Monoclonal antibody M00074 available?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Smad4 Rabbit Monoclonal antibody M00074 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-22
Question
Would anti-Smad4 Rabbit Monoclonal antibody M00074 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
According to the expression profile of kidney, SMAD4 is highly expressed in kidney. So, it is likely that anti-Smad4 Rabbit Monoclonal antibody M00074 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-02-19
Question
My question regarding product M00074, anti-Smad4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00074 anti-Smad4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-09
Question
I was wanting to use your anti-Smad4 Rabbit Monoclonal antibody for WB for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
As indicated on the product datasheet, M00074 anti-Smad4 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-02
Question
We have seen staining in human muscle. Are there any suggestions? Is anti-Smad4 Rabbit Monoclonal antibody supposed to stain muscle positively?
K. Miller
Verified customer
Asked: 2018-07-16
Answer
From what I have seen in literature muscle does express SMAD4. From what I have seen in Uniprot.org, SMAD4 is expressed in kidney, fetal brain, placenta, muscle, liver, among other tissues. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2018-07-16
Question
Will anti-Smad4 Rabbit Monoclonal antibody M00074 work on pig IF with fetal brain?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
Our lab technicians have not tested anti-Smad4 Rabbit Monoclonal antibody M00074 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Smad4 Rabbit Monoclonal antibody M00074 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig fetal brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-28
Question
We ordered your anti-Smad4 Rabbit Monoclonal antibody for WB on fetal brain in a previous experiment. I am using human, and We intend to use the antibody for IHC next. Our lab want to know about examining fetal brain as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
I looked at the website and datasheets of our anti-Smad4 Rabbit Monoclonal antibody and it seems that M00074 has been validated on human in both WB and IHC. Thus M00074 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-11-02
Question
Is this M00074 anti-Smad4 Rabbit Monoclonal antibody reactive to the isotypes of SMAD4?
B. Collins
Verified customer
Asked: 2017-07-06
Answer
The immunogen of M00074 anti-Smad4 Rabbit Monoclonal antibody is A synthesized peptide derived from human Smad4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-06
Question
My team were satisfied with the WB result of your anti-Smad4 Rabbit Monoclonal antibody. However we have been able to see positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD4 supposed to be expressed?
B. Carter
Verified customer
Asked: 2014-07-15
Answer
Based on literature, kidney does express SMAD4. Generally SMAD4 expresses in cytoplasm. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2014-07-15
Question
See attached the WB image, lot number and protocol we used for kidney using anti-Smad4 Rabbit Monoclonal antibody M00074. Please let me know if you require anything else.
M. Collins
Verified customer
Asked: 2013-06-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-04
