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Product Info Summary
| SKU: | M00074-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277
SKU/Catalog Number
M00074-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 (Catalog # M00074-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00074-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM277
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Smad4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Smad4. This antibody may also react to mouse or rat Smad4, as predicted by immunogen homology.
Reactive Species
M00074-1 is reactive to SMAD4 in Human
Reconstitution
Calculated molecular weight
60439 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00074-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2000 dilution
WB: 1:500-1:1000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of Jurkat and IMR32 cells lysates using Anti-Smad4 Rabbit Monoclonal Antibody (Clone RM277) at a 1:500 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smad4 Rabbit Monoclonal Antibody (Clone RM277) at a 1:2000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SMAD4 (Source: Uniprot.org, NCBI)
Gene Name
SMAD4
Full Name
Mothers against decapentaplegic homolog 4
Weight
60439 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;Deletion target in pancreatic carcinoma 4;SMAD family member 4;SMAD 4;Smad4;hSMAD4;SMAD4;DPC4, MADH4; SMAD4 DPC4, JIP, MADH4, MYHRS SMAD family member 4 mothers against decapentaplegic homolog 4|MAD homolog 4|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|deletion target in pancreatic carcinoma 4|mothers against decapentaplegic, Drosophila, homolog of, 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD4, check out the SMAD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 (M00074-1)
Hello CJ!
M00074-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
TGF%u2010%u03B2 regulates the proliferation of lung adenocarcinoma cells by inhibiting PIK3R3 expression
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277
Question
We are currently using anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 (M00074-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-09
Question
Is a blocking peptide available for product anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 (M00074-1)?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We do provide the blocking peptide for product anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 (M00074-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-05
Question
I was wanting to use your anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 for IHC for rat muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat muscle identification?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
As indicated on the product datasheet, M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
I see that the anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 works with IHC, what is the protocol used to produce the result images on the product page?
E. Johnson
Verified customer
Asked: 2019-09-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-04
Question
We ordered your anti-Smad4 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
I have checked the website and datasheets of our anti-Smad4 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-07-10
Question
Is this M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 reactive to the isotypes of SMAD4?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The immunogen of M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 is A peptide corresponding to the C-terminus of human Smad4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-28
Question
Do you have a BSA free version of anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 available?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-17
Question
Our lab want to know about using your anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 for regulation of transforming growth factor beta receptor signaling pathway studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
Thank you for your inquiry. This M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 is tested on imr32 cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-10
Question
My colleagues were happy with the WB result of your anti-Smad4 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-03-19
Answer
Based on literature, placenta does express SMAD4. Generally SMAD4 expresses in cytoplasm. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2019-03-19
Question
Does anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 work for IHC with muscle?
S. Li
Verified customer
Asked: 2019-03-13
Answer
According to the expression profile of muscle, SMAD4 is highly expressed in muscle. So, it is likely that anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 will work for IHC with muscle.
Boster Scientific Support
Answered: 2019-03-13
Question
Would M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
As indicated on the product datasheet, M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-07
Question
Please see the WB image, lot number and protocol we used for muscle using anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle using anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1. Let me know if you need anything else.
N. Krishna
Verified customer
Asked: 2015-07-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-14
Question
Can you help my question with product M00074-1, anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Bhatt
Verified customer
Asked: 2014-07-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00074-1 anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-07-11
Question
We want to test anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 on rat muscle for research purposes, then I may be interested in using anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Jones
Verified customer
Asked: 2014-07-02
Answer
The products we sell, including anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 M00074-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-07-02
Question
We have seen staining in rat liver. What should we do? Is anti-Smad4 Rabbit Monoclonal antibody, Clone#RM277 supposed to stain liver positively?
S. Evans
Verified customer
Asked: 2013-05-13
Answer
From literature liver does express SMAD4. From Uniprot.org, SMAD4 is expressed in kidney, fetal brain, placenta, muscle, liver, among other tissues. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2013-05-13
