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Product Info Summary
| SKU: | A00728S465 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Smad1 (phospho-S465) Antibody
SKU/Catalog Number
A00728S465
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Smad1 (phospho-S465) Antibody catalog # A00728S465. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad1 (phospho-S465) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00728S465)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 465.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00728S465 is reactive to SMAD1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
52260 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00728S465 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of SMAD1 using Anti-Smad1 (phospho-S465) Antibody (A00728S465).
Immunohistochemistry (IHC) analyzes of p-Smad1 (S465) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Smad1 (phospho-S465) Antibody A00728S465
Western blot (WB) analysis of p-Smad1 (S465) pAb at 1:500 dilutionLane1:A549 whole cell lysate(40ug)
Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate(40ug)
Lane3:A549 treated with UV for 5 minutes then repair for 3 hours whole cell lysate(40ug)
Lane4:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug)
Lane5:A549 treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug)
Lane6:The Uterus tissue lysate of Mouse(40ug)
Lane7:The Uterus tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SMAD1 (Source: Uniprot.org, NCBI)
Gene Name
SMAD1
Full Name
Mothers against decapentaplegic homolog 1
Weight
52260 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 1;MAD homolog 1;Mothers against DPP homolog 1;JV4-1;Mad-related protein 1;SMAD family member 1;SMAD 1;Smad1;hSMAD1;Transforming growth factor-beta-signaling protein 1;BSP-1;SMAD1;BSP1, MADH1, MADR1; SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD1, check out the SMAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad1 (phospho-S465) Antibody (A00728S465)
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