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Product Info Summary
| SKU: | PA2114 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Smad1 Antibody Picoband®
SKU/Catalog Number
PA2114
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Smad1 Antibody catalog # PA2114. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2114)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Smad1, different from the related rat sequence by one amino acid, and different from the related human sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2114 is reactive to Smad1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
52157 MW
Background of SMAD1
Mothers against decapentaplegic homolog 1 also known as SMAD family member 1 or SMAD1 is a protein that in humans is encoded by the SMAD1 gene. SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene "mothers against decapentaplegic" (Mad) and the C. elegans gene Sma. The gene was assigned to human chromosome 4q31.21. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2114 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: SMMC Cell, K562 Cell, HT1080 Cell, HELA Cell, JURKAT Cell,
IHC: Human Lung Cancer Tissue
ICC: A549 Cell
Validation Images & Assay Conditions
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Anti-Smad1 antibody, PA2114, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: K562 Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: JURKAT Cell Lysate
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Anti-Smad1 antibody, PA2114, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-Smad1 antibody, PA2114, ICC
ICC: A549 Cell
Protein Target Info & Infographic
Gene/Protein Information For Smad1 (Source: Uniprot.org, NCBI)
Gene Name
Smad1
Full Name
Mothers against decapentaplegic homolog 1
Weight
52157 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 1;MAD homolog 1;Mothers against DPP homolog 1;Dwarfin-A;Dwf-A;Mothers-against-DPP-related 1;Mad-related protein 1;mMad1;SMAD family member 1;SMAD 1;Smad1;Smad1;Madh1, Madr1; SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Smad1, check out the Smad1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Smad1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad1 Antibody Picoband® (PA2114)
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No publications found for PA2114
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Customer Q&As
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17 Customer Q&As for Anti-Smad1 Antibody Picoband®
Question
Would anti-Smad1 antibody PA2114 work for ICC with ovarian carcinoma?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
According to the expression profile of ovarian carcinoma, SMAD1 is highly expressed in ovarian carcinoma. So, it is likely that anti-Smad1 antibody PA2114 will work for ICC with ovarian carcinoma.
Boster Scientific Support
Answered: 2020-03-31
Question
Is there a BSA free version of anti-Smad1 antibody PA2114 available?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Smad1 antibody PA2114 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-30
Question
We are currently using anti-Smad1 antibody PA2114 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
J. Carter
Verified customer
Asked: 2020-02-11
Answer
The anti-Smad1 antibody (PA2114) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-11
Question
Does anti-Smad1 antibody PA2114 work on primate ICC with mammary carcinoma?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
Our lab technicians have not validated anti-Smad1 antibody PA2114 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Smad1 antibody PA2114 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate mammary carcinoma in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-30
Question
I am looking for using your anti-Smad1 antibody for cellular response to bmp stimulus studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
T. Walker
Verified customer
Asked: 2019-11-11
Answer
We appreciate your inquiry. This PA2114 anti-Smad1 antibody is validated on smmc cell lysate, k562 cell lysate, ht1080 cell lysate, hela cell lysate, jurkat cell lysate, lung cancer tissue. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-11
Question
I would like to test anti-Smad1 antibody PA2114 on rat ovarian carcinoma for research purposes, then I may be interested in using anti-Smad1 antibody PA2114 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Carter
Verified customer
Asked: 2019-11-08
Answer
The products we sell, including anti-Smad1 antibody PA2114, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-08
Question
We bought anti-Smad1 antibody for WB on kidney in the past. I am using human, and We want to use the antibody for IHC next. I would like examining kidney as well as uterus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
I viewed the website and datasheets of our anti-Smad1 antibody and I see that PA2114 has been validated on human in both WB and IHC. Thus PA2114 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-15
Question
Please see the WB image, lot number and protocol we used for ovarian carcinoma using anti-Smad1 antibody PA2114. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
I was wanting to use your anti-Smad1 antibody for ICC for rat ovarian carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat ovarian carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
You can see on the product datasheet, PA2114 anti-Smad1 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat ovarian carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-24
Question
I have a question about product PA2114, anti-Smad1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2114 anti-Smad1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-18
Question
Is a blocking peptide available for product anti-Smad1 antibody (PA2114)?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
We do provide the blocking peptide for product anti-Smad1 antibody (PA2114). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovarian carcinoma using anti-Smad1 antibody PA2114. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-13
Question
Is this PA2114 anti-Smad1 antibody reactive to the isotypes of SMAD1?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
The immunogen of PA2114 anti-Smad1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Smad1(253-267aa AEISRGDVQAVAYEE), different from the related rat sequence by one amino acid, and different from the related human sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-04
Question
We have seen staining in human kidney. What should we do? Is anti-Smad1 antibody supposed to stain kidney positively?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
From literature kidney does express SMAD1. From Uniprot.org, SMAD1 is expressed in visceral pleura, kidney, mammary carcinoma, placenta, uterus, ovarian carcinoma, among other tissues. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2017-09-26
Question
I see that the anti-Smad1 antibody PA2114 works with ICC, what is the protocol used to produce the result images on the product page?
B. Parker
Verified customer
Asked: 2016-09-08
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-09-08
Question
Does PA2114 anti-Smad1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Singh
Verified customer
Asked: 2013-11-27
Answer
As indicated on the product datasheet, PA2114 anti-Smad1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-11-27
Question
Our lab were satisfied with the WB result of your anti-Smad1 antibody. However we have seen positive staining in uterus cytoplasm. using this antibody. Is that expected? Could you tell me where is SMAD1 supposed to be expressed?
R. Collins
Verified customer
Asked: 2013-05-17
Answer
According to literature, uterus does express SMAD1. Generally SMAD1 expresses in cytoplasm. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2013-05-17
