Click image to see more details
Product Info Summary
| SKU: | A00969 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NFAT1/NFATC2 Antibody Picoband®
View all NFATC2/NFAT1 Antibodies
SKU/Catalog Number
A00969
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFAT1/NFATC2 Antibody Picoband® catalog # A00969. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFAT1/NFATC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00969)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human NFAT1 recombinant protein (Position: Q594-H676).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00969 is reactive to NFATC2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
100.146kDa
Background of NFATC2/NFAT1
NFATC2 (Nuclear factor of activated T-cells, cytoplasmic 2), also known as NFATP or the 'preexisting component' of NFAT, is present in the cytosolic fraction of unstimulated T cells, which is also a member of the nuclear factor of activated T cells (NFAT) family. The NFATC2 gene is mapped on 20q13.2. NFATC2 is highly homologous to NFATC1 over a limited domain which shows similarity to the Dorsal/Rel family but has a wider tissue distribution. Ectopic expression of NFATC2 inhibited the basal activity of the human CDK4 promoter. Additionally, both Calna-/- and Nfatc2 -/- mice had elevated protein levels of Cdk4, confirming a negative regulatory role for the calcineurin/NFAT pathway. NFATC2 controls myoblast fusion at a specific stage of myogenesis after the initial formation of a myotube and is necessary for further cell growth. Overexpression of NFATC2 promoted differentiation of osteoclast precursor cells into tartrate-resistant acid phosphatase-positive (TRAP-positive) multinucleated osteoclast-like cells even in the absence of RANKL.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00969 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human K562 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NFAT1 using anti-NFAT1 antibody (A00969).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT1 antigen affinity purified polyclonal antibody (Catalog # A00969) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT1 at approximately 100KD. The expected band size for NFAT1 is at 100KD.
Protein Target Info & Infographic
Gene/Protein Information For NFATC2 (Source: Uniprot.org, NCBI)
Gene Name
NFATC2
Full Name
Nuclear factor of activated T-cells, cytoplasmic 2
Weight
100.146kDa
Alternative Names
Nuclear factor of activated T-cells, cytoplasmic 2; NF-ATc2; NFATc2; NFAT pre-existing subunit; NF-ATp; T-cell transcription factor NFAT1; NFATC2; NFAT1, NFATP; NFATC2 NFAT1, NFATP nuclear factor of activated T cells 2 nuclear factor of activated T-cells, cytoplasmic 2|NF-ATc2|NFAT pre-existing subunit|NFAT transcription complex, preexisting component|T cell transcription factor NFAT1|nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2|nuclear factor of activated T-cells, preexisting component|preexisting nuclear factor of activated T-cells 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFATC2, check out the NFATC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFATC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFAT1/NFATC2 Antibody Picoband® (A00969)
Hello CJ!
A00969 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bai, Y., Li, Z.X., Wang, H.L., Lian, G.C., & Wang, Y. (2017). The protective effects of PCPA against monocrotaline-induced pulmonary arterial hypertension are mediated through the downregulation of NFAT-1 and NF-κB. International Journal of Molec...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NFAT1/NFATC2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-NFAT1/NFATC2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-NFAT1/NFATC2 Antibody Picoband®
Question
We are currently using anti-NFAT1/NFATC2 antibody A00969 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
The anti-NFAT1/NFATC2 antibody (A00969) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-01
