Anti-SLP2/SYTL2 Antibody Picoband™

Product Name

Anti-SLP2/SYTL2 Antibody Picoband™

View all SYTL2 Antibodies

SKU/Catalog Number

A08255-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLP2/SYTL2 Antibody Picoband™ catalog # A08255-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SLP2/SYTL2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08255-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SLP2/SYTL2 recombinant protein (Position: Q522-K934).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08255-1 is reactive to SYTL2 in Human

Applications

A08255-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

120-130 kDa

Calculated molecular weight

Background of SYTL2

Synaptotagmin-like 2, also known as SYTL2, is a human gene. The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SLP2/SYTL2 using anti-SLP2/SYTL2 antibody (A08255-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLP2/SYTL2 antigen affinity purified polyclonal antibody (Catalog # A08255-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLP2/SYTL2 at approximately 120-130 kDa. The expected band size for SLP2/SYTL2 is at 105 kDa.

Gene/Protein Information For SYTL2 (Source: Uniprot.org, NCBI)

Alternative Names

Synaptotagmin-like protein 2 SYTL2 CHR11SYT, EXO4, PPP1R151, SGA72M, SLP2, SLP2A synaptotagmin like 2 synaptotagmin-like protein 2|breast cancer-associated antigen SGA-72M|chromosome 11 synaptotagmin|exophilin-4|protein phosphatase 1, regulatory subunit 151

For more info on SYTL2, check out the SYTL2 Infographic

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In this infographic, you will see the following information for SYTL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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