Anti-SLC7A5 Antibody (N-Term)

Product Name

Anti-SLC7A5 Antibody (N-Term)

View all SLC7A5/LAT1 Antibodies

SKU/Catalog Number

M01016-1

Size

50 µl

Description

Boster Bio Anti-SLC7A5 Antibody (N-Term) (Catalog # M01016-1). Tested in WB, IF application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-SLC7A5 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01016-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This SLC7A5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from human SLC7A5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01016-1 is reactive to SLC7A5 in Human

Applications

M01016-1 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55010 Da

Background of SLC7A5/LAT1

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood- brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L- leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:2000

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling SLC7A5 with M01016-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

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All lanes : Anti-SLC7A5 Antibody (N-Term) at 1:2000 dilution
Lane 1: Human lung lysate
Lane 2: Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 55 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For SLC7A5 (Source: Uniprot.org, NCBI)

Alternative Names

4F2 LC; 4F2LC; CD98; CD98lc; D16S469EL-type amino acid transporter 1; E16; Integral membrane protein E16; large neutral amino acids transporter 1; large neutral amino acids transporter small subunit 1; LAT1; LAT1hLAT1; MPE16; MPE16CD98 light chain; SLC7A5; sodium-independent neutral amino acid transporter LAT1,4F2LC; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5,4F2 light chain; Solute carrier family 7 member 5; TA1; y+ system cationic amino acid transporter SLC7A5 4F2LC, CD98, D16S469E, E16, LAT1, MPE16 solute carrier family 7 member 5 large neutral amino acids transporter small subunit 1|4F2 light chain|CD98 light chain|L-type amino acid transporter 1|integral membrane protein E16|sodium-independent neutral amino acid transporter LAT1|solute carrier family 7 (amino acid transporter light chain, L system), member 5|solute carrier family 7 (cationic amino acid transporter, y+ system), member 5

For more info on SLC7A5, check out the SLC7A5 Infographic

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In this infographic, you will see the following information for SLC7A5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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