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Product Info Summary
| SKU: | A06872-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SLC10A1/NTCP1 Antibody Picoband®
SKU/Catalog Number
A06872-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC10A1/NTCP1 Antibody Picoband® catalog # A06872-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SLC10A1/NTCP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06872-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLC10A1/NTCP1 recombinant protein (Position: L137-E328).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06872-2 is reactive to SLC10A1 in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
38.119kDa
Background of SLC10A1
Sodium/bile acid cotransporter also known as the Na+-taurocholate cotransporting polypeptide (NTCP) or liver bile acid transporter (LBAT) is a protein that in humans is encoded by the SLC10A1 (solute carrier family 10 member 1) gene. The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06872-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: monkey liver tissue, human HCCP tissue, rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC10A1/NTCP1 using anti-SLC10A1/NTCP1 antibody (A06872-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey liver tissue lysates,
Lane 2: human HCCP tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1/NTCP1 antigen affinity purified polyclonal antibody (Catalog # A06872-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1/NTCP1 at approximately 38 kDa. The expected band size for SLC10A1/NTCP1 is at 38 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SLC10A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC10A1
Full Name
Sodium/bile acid cotransporter
Weight
38.119kDa
Superfamily
bile acid:sodium symporter (BASS) (TC 2.A.28) family
Alternative Names
Sodium/bile acid cotransporter; Na (+)/bile acid cotransporter; Na (+)/taurocholate transport protein; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1; Slc10a1; Ntcp SLC10A1 NTCP solute carrier family 10 member 1 sodium/bile acid cotransporter|Na(+)/bile acid cotransporter|Na(+)/taurocholate transport protein|Na/taurocholate cotransporting polypeptide|cell growth-inhibiting gene 29 protein|growth-inhibiting protein 29|sodium/taurocholate cotransporter|sodium/taurocholate cotransporting polypeptide|solute carrier family 10 (sodium/bile acid cotransporter family), member 1|solute carrier family 10 (sodium/bile acid cotransporter), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC10A1, check out the SLC10A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC10A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC10A1/NTCP1 Antibody Picoband® (A06872-2)
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