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Product Info Summary
| SKU: | CI1031 |
|---|---|
| Size: | 150μg/50μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ChIP, IF, WB |
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Product info
Product Name
Anti-Ski3 TTC37 Antibody
SKU/Catalog Number
CI1031
Size
150μg/50μl
Form
Liquid
Description
Boster Bio Anti-Ski3 TTC37 Antibody (Catalog# CI1031). Tested in WB, IF, ChIP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Cite This Product
Anti-Ski3 TTC37 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1031)
Host
Rabbit
Contents
Protein G purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Clonality
Polyclonal
Immunogen
This antibody is raised in rabbit against human SKI3, using a recombinant protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
CI1031 is reactive to TTC37 in Human
Reconstitution
Observed Molecular Weight
Calculated molecular weight
175486 MW
Background of TTC37
SKI3 is a component of the SKI complex which is composed of SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, making the link between the SKI complex and the exosome in order to perform mRNA degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
CI1031 is guaranteed for ChIP, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Positive Control
Validation Images & Assay Conditions
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Western blot analysis of SKI3 expression in nuclear extracts from HeLa cells (lane 1) and the purified human SKI complex (lane 2). SKI3 at 150KD was detected using Anti-SKI3 polyclonal antibody (Catalog # CI1031) at 1/1000 dilution.
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Western blot analysis of SKI3 expression in nuclear extracts from HeLa cells (20
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Immunofluorescence images stained on HeLa cells: (Left) Nuclei stained with DAPI. (Middle) Cells stained with anti-SKI3 polyclonal antibody (Catalog # CI1031). (Right) Merged images of two stains from the left and middle.
Protein Target Info & Infographic
Gene/Protein Information For TTC37 (Source: Uniprot.org, NCBI)
Gene Name
TTC37
Full Name
Tetratricopeptide repeat protein 37
Weight
175486 MW
Alternative Names
tetratricopeptide repeat domain 37 TTC37 KIAA0372, Ski3, THES tetratricopeptide repeat domain 37 tetratricopeptide repeat protein 37|SKI3 homolog|TPR repeat protein 37|thespin|tricho-hepatic-enteric syndrome protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTC37, check out the TTC37 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTC37: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ski3 TTC37 Antibody (CI1031)
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No publications found for CI1031
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Customer Q&As
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6 Customer Q&As for Anti-Ski3 TTC37 Antibody
Question
Is this CI1031 anti-Ski3 antibody reactive to the isotypes of TTC37?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The immunogen of CI1031 anti-Ski3 antibody is This antibody is raised in rabbit against human SKI3, using a recombinant protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-29
Question
Will anti-Ski3 antibody CI1031 work on zebrafish ChIP with lung carcinoma melanoma?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
Our lab technicians have not tested anti-Ski3 antibody CI1031 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Ski3 antibody CI1031 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lung carcinoma melanoma in ChIP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-Ski3 antibody CI1031 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The anti-Ski3 antibody (CI1031) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-04
Question
My lab would like to test anti-Ski3 antibody CI1031 on human lung carcinoma melanoma for research purposes, then I may be interested in using anti-Ski3 antibody CI1031 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The products we sell, including anti-Ski3 antibody CI1031, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-21
Question
Is a blocking peptide available for product anti-Ski3 antibody (CI1031)?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
We do provide the blocking peptide for product anti-Ski3 antibody (CI1031). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-01
Question
I have a question about product CI1031, anti-Ski3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Johnson
Verified customer
Asked: 2013-07-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free CI1031 anti-Ski3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-09
