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Product Info Summary
| SKU: | A05725-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-P2RY5/LPAR6 Antibody Picoband®
View all LPAR6/P2RY5 Antibodies
SKU/Catalog Number
A05725-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-P2RY5/LPAR6 Antibody Picoband® catalog # A05725-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P2RY5/LPAR6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05725-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human P2RY5, which shares 92.3% and 89.7% amino acid (aa) sequence identity with mouse and rat P2RY5, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05725-1 is reactive to LPAR6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
101272 MW
Background of LPAR6/P2RY5
Lysophosphatidic acid receptor 6 also known as LPA6, P2RY5, and GPR87, is a protein that in humans is encoded by the LPAR6 gene. The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. This gene aligns with an internal intron of the retinoblastoma susceptibility gene in the reverse orientation. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05725-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human HepG2 whole cell, human RT4 whole cell, human Hacat whole cell, human T47D whole cell, human A549 whole cell
FCM: A549 cell, PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of P2RY5 using anti-P2RY5 antibody (A05725-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human T47D whole cell lysates,
Lane 5: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RY5 antigen affinity purified polyclonal antibody (Catalog # A05725-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RY5 at approximately 39 kDa. The expected band size for P2RY5 is at 39 kDa.
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Figure 2. Flow Cytometry analysis of A549 cells using anti-P2RY5 antibody (A05725-1).
Overlay histogram showing A549 cells stained with A05725-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P2RY5 Antibody (A05725-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-P2RY5 antibody (A05725-1).
Overlay histogram showing PC-3 cells stained with A05725-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P2RY5 Antibody (A05725-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For LPAR6 (Source: Uniprot.org, NCBI)
Gene Name
LPAR6
Full Name
Lysophosphatidic acid receptor 6
Weight
101272 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Lysophosphatidic acid receptor 6; LPA receptor 6; LPA-6; Oleoyl-L-alpha-lysophosphatidic acid receptor; P2Y purinoceptor 5; P2Y5; Purinergic receptor 5; RB intron encoded G-protein coupled receptor; LPAR6; P2RY5 LPAR6 ARWH1, HYPT8, LAH3, LPA-6, P2RY5, P2Y5 lysophosphatidic acid receptor 6 lysophosphatidic acid receptor 6|G-protein coupled purinergic receptor P2Y5|LPA receptor 6|P2Y purinoceptor 5|RB intron encoded G-protein coupled receptor|oleoyl-L-alpha-lysophosphatidic acid receptor|purinergic receptor 5|purinergic receptor P2Y G protein-coupled protein 5|purinergic receptor P2Y, G-protein coupled, 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LPAR6, check out the LPAR6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LPAR6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-P2RY5/LPAR6 Antibody Picoband® (A05725-1)
Hello CJ!
A05725-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid
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Customer Q&As
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3 Customer Q&As for Anti-P2RY5/LPAR6 Antibody Picoband®
Question
Is a blocking peptide available for product anti-P2RY5/LPAR6 antibody (A05725-1)?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
We do provide the blocking peptide for product anti-P2RY5/LPAR6 antibody (A05725-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-14
Question
We are currently using anti-P2RY5/LPAR6 antibody A05725-1 for mouse tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-P2RY5/LPAR6 antibody (A05725-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
Question
Is there a BSA free version of anti-P2RY5/LPAR6 antibody A05725-1 available?
D. Yang
Verified customer
Asked: 2014-04-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-P2RY5/LPAR6 antibody A05725-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-04-23
