Anti-Siglec11 Antibody

Product Name

Anti-Siglec11 Antibody

View all Siglec-11 Antibodies

SKU/Catalog Number

A12601

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Siglec11 Antibody (Catalog # A12601). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

Siglec11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Siglec11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12601)

Host

Rabbit

Contents

Siglec11 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Siglec11 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11. The immunogen is located within the last 50 amino acids of Siglec11.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.

Reactive Species

A12601 is reactive to SIGLEC11 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

75795 MW

Background of Siglec-11

Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12601 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Siglec11 antibody can be used for detection of Siglec11 by Western blot at 1 μg/mL. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For SIGLEC11 (Source: Uniprot.org, NCBI)

Alternative Names

Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11 SIGLEC11 sialic acid binding Ig like lectin 11 sialic acid-binding Ig-like lectin 11|siglec-11

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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